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Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease

Decreased diaphragm function is a crucial factor leading to reduced ventilatory efficiency and worsening of quality of life in chronic obstructive pulmonary disease (COPD). Exercise training has been demonstrated to effectively improve the function of the diaphragm. However, the mechanism of this pr...

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Autores principales: Li, Jian, Lu, Yufan, Li, Ning, Li, Peijun, Su, Jianqing, Wang, Zhengrong, Wang, Ting, Yang, Zhaoyu, Yang, Yahui, Chen, Haixia, Xiao, Lu, Duan, Hongxia, Wu, Weibing, Liu, Xiaodan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169662/
https://www.ncbi.nlm.nih.gov/pubmed/32186768
http://dx.doi.org/10.3892/ijmm.2020.4537
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author Li, Jian
Lu, Yufan
Li, Ning
Li, Peijun
Su, Jianqing
Wang, Zhengrong
Wang, Ting
Yang, Zhaoyu
Yang, Yahui
Chen, Haixia
Xiao, Lu
Duan, Hongxia
Wu, Weibing
Liu, Xiaodan
author_facet Li, Jian
Lu, Yufan
Li, Ning
Li, Peijun
Su, Jianqing
Wang, Zhengrong
Wang, Ting
Yang, Zhaoyu
Yang, Yahui
Chen, Haixia
Xiao, Lu
Duan, Hongxia
Wu, Weibing
Liu, Xiaodan
author_sort Li, Jian
collection PubMed
description Decreased diaphragm function is a crucial factor leading to reduced ventilatory efficiency and worsening of quality of life in chronic obstructive pulmonary disease (COPD). Exercise training has been demonstrated to effectively improve the function of the diaphragm. However, the mechanism of this process has not been identified. The emergence of metabolomics has allowed the exploration of new ideas. The present study aimed to analyze the potential biomarkers of exercise-dependent enhancement of diaphragm function in COPD using metabolomics. Sprague Dawley rats were divided into three groups: COPD + exercise group (CEG); COPD model group (CMG); and control group (CG). The first two groups were exposed to cigarette smoke for 16 weeks to establish a COPD model. Then, the rats in the CEG underwent aerobic exercise training for 9 weeks. Following confirmation that exercise effectively improved the diaphragm function, a gas chromatography tandem time-of-flight mass spectrometry analysis system was used to detect the differential metabolites and associated pathways in the diaphragm muscles of the different groups. Following exercise intervention, the pulmonary function and diaphragm contractility of the CEG rats were significantly improved compared with those of the CMG rats. A total of 36 different metabolites were identified in the comparison between the CMG and the CG. Pathway enrichment analysis indicated that these different metabolites were involved in 17 pathways. A total of 29 different metabolites were identified in the comparison between the CMG and the CEG, which are involved in 14 pathways. Candidate biomarkers were selected, and the pathways analysis of these metabolites demonstrated that 2 types of metabolic pathways, the nicotinic acid and nicotinamide metabolism and arginine and proline metabolism pathways, were associated with exercise-induced pulmonary rehabilitation.
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spelling pubmed-71696622020-04-24 Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease Li, Jian Lu, Yufan Li, Ning Li, Peijun Su, Jianqing Wang, Zhengrong Wang, Ting Yang, Zhaoyu Yang, Yahui Chen, Haixia Xiao, Lu Duan, Hongxia Wu, Weibing Liu, Xiaodan Int J Mol Med Articles Decreased diaphragm function is a crucial factor leading to reduced ventilatory efficiency and worsening of quality of life in chronic obstructive pulmonary disease (COPD). Exercise training has been demonstrated to effectively improve the function of the diaphragm. However, the mechanism of this process has not been identified. The emergence of metabolomics has allowed the exploration of new ideas. The present study aimed to analyze the potential biomarkers of exercise-dependent enhancement of diaphragm function in COPD using metabolomics. Sprague Dawley rats were divided into three groups: COPD + exercise group (CEG); COPD model group (CMG); and control group (CG). The first two groups were exposed to cigarette smoke for 16 weeks to establish a COPD model. Then, the rats in the CEG underwent aerobic exercise training for 9 weeks. Following confirmation that exercise effectively improved the diaphragm function, a gas chromatography tandem time-of-flight mass spectrometry analysis system was used to detect the differential metabolites and associated pathways in the diaphragm muscles of the different groups. Following exercise intervention, the pulmonary function and diaphragm contractility of the CEG rats were significantly improved compared with those of the CMG rats. A total of 36 different metabolites were identified in the comparison between the CMG and the CG. Pathway enrichment analysis indicated that these different metabolites were involved in 17 pathways. A total of 29 different metabolites were identified in the comparison between the CMG and the CEG, which are involved in 14 pathways. Candidate biomarkers were selected, and the pathways analysis of these metabolites demonstrated that 2 types of metabolic pathways, the nicotinic acid and nicotinamide metabolism and arginine and proline metabolism pathways, were associated with exercise-induced pulmonary rehabilitation. D.A. Spandidos 2020-06 2020-03-12 /pmc/articles/PMC7169662/ /pubmed/32186768 http://dx.doi.org/10.3892/ijmm.2020.4537 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Jian
Lu, Yufan
Li, Ning
Li, Peijun
Su, Jianqing
Wang, Zhengrong
Wang, Ting
Yang, Zhaoyu
Yang, Yahui
Chen, Haixia
Xiao, Lu
Duan, Hongxia
Wu, Weibing
Liu, Xiaodan
Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
title Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
title_full Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
title_fullStr Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
title_full_unstemmed Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
title_short Muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
title_sort muscle metabolomics analysis reveals potential biomarkers of exercise-dependent improvement of the diaphragm function in chronic obstructive pulmonary disease
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169662/
https://www.ncbi.nlm.nih.gov/pubmed/32186768
http://dx.doi.org/10.3892/ijmm.2020.4537
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