Cargando…

Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato

Copper‐based antimicrobial compounds are widely and historically used to control plant diseases, such as late blight caused by Phytophthora infestans, which seriously affects the yield and quality of potato. We previously identified that copper ion (Cu(2+)) acts as an extremely sensitive elicitor to...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Hai‐Feng, Xue, Xiao‐Jing, Yu, Yue, Xu, Ming‐Ming, Lu, Chong‐Chong, Meng, Xuan‐Lin, Zhang, Bao‐Gang, Ding, Xin‐Hua, Chu, Zhao‐Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170774/
https://www.ncbi.nlm.nih.gov/pubmed/32077242
http://dx.doi.org/10.1111/mpp.12919
_version_ 1783523941302665216
author Liu, Hai‐Feng
Xue, Xiao‐Jing
Yu, Yue
Xu, Ming‐Ming
Lu, Chong‐Chong
Meng, Xuan‐Lin
Zhang, Bao‐Gang
Ding, Xin‐Hua
Chu, Zhao‐Hui
author_facet Liu, Hai‐Feng
Xue, Xiao‐Jing
Yu, Yue
Xu, Ming‐Ming
Lu, Chong‐Chong
Meng, Xuan‐Lin
Zhang, Bao‐Gang
Ding, Xin‐Hua
Chu, Zhao‐Hui
author_sort Liu, Hai‐Feng
collection PubMed
description Copper‐based antimicrobial compounds are widely and historically used to control plant diseases, such as late blight caused by Phytophthora infestans, which seriously affects the yield and quality of potato. We previously identified that copper ion (Cu(2+)) acts as an extremely sensitive elicitor to induce ethylene (ET)‐dependent immunity in Arabidopsis. Here, we found that Cu(2+) induces the defence response to P. infestans in potato. Cu(2+) suppresses the transcription of the abscisic acid (ABA) biosynthetic genes StABA1 and StNCED1, resulting in decreased ABA content. Treatment with ABA or inhibitor fluridone made potato more susceptible or resistance to late blight, respectively. In addition, potato with knockdown of StABA1 or StNCED1 showed greater resistance to late blight, suggesting that ABA negatively regulates potato resistance to P. infestans. Cu(2+) also promotes the rapid biosynthesis of ET. Potato plants treated with 1‐aminocyclopropane‐1‐carboxylate showed enhanced resistance to late blight. Repressed expression of StEIN2 or StEIN3 resulted in enhanced transcription of StABA1 and StNCED1, accumulation of ABA and susceptibility to P. infestans. Consistently, StEIN3 directly binds to the promoter regions of StABA1 and StNCED1. Overall, we concluded that Cu(2+) triggers the defence response to potato late blight by activating ET biosynthesis to inhibit the biosynthesis of ABA.
format Online
Article
Text
id pubmed-7170774
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-71707742020-04-21 Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato Liu, Hai‐Feng Xue, Xiao‐Jing Yu, Yue Xu, Ming‐Ming Lu, Chong‐Chong Meng, Xuan‐Lin Zhang, Bao‐Gang Ding, Xin‐Hua Chu, Zhao‐Hui Mol Plant Pathol Original Articles Copper‐based antimicrobial compounds are widely and historically used to control plant diseases, such as late blight caused by Phytophthora infestans, which seriously affects the yield and quality of potato. We previously identified that copper ion (Cu(2+)) acts as an extremely sensitive elicitor to induce ethylene (ET)‐dependent immunity in Arabidopsis. Here, we found that Cu(2+) induces the defence response to P. infestans in potato. Cu(2+) suppresses the transcription of the abscisic acid (ABA) biosynthetic genes StABA1 and StNCED1, resulting in decreased ABA content. Treatment with ABA or inhibitor fluridone made potato more susceptible or resistance to late blight, respectively. In addition, potato with knockdown of StABA1 or StNCED1 showed greater resistance to late blight, suggesting that ABA negatively regulates potato resistance to P. infestans. Cu(2+) also promotes the rapid biosynthesis of ET. Potato plants treated with 1‐aminocyclopropane‐1‐carboxylate showed enhanced resistance to late blight. Repressed expression of StEIN2 or StEIN3 resulted in enhanced transcription of StABA1 and StNCED1, accumulation of ABA and susceptibility to P. infestans. Consistently, StEIN3 directly binds to the promoter regions of StABA1 and StNCED1. Overall, we concluded that Cu(2+) triggers the defence response to potato late blight by activating ET biosynthesis to inhibit the biosynthesis of ABA. John Wiley and Sons Inc. 2020-01-30 /pmc/articles/PMC7170774/ /pubmed/32077242 http://dx.doi.org/10.1111/mpp.12919 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Liu, Hai‐Feng
Xue, Xiao‐Jing
Yu, Yue
Xu, Ming‐Ming
Lu, Chong‐Chong
Meng, Xuan‐Lin
Zhang, Bao‐Gang
Ding, Xin‐Hua
Chu, Zhao‐Hui
Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato
title Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato
title_full Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato
title_fullStr Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato
title_full_unstemmed Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato
title_short Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato
title_sort copper ions suppress abscisic acid biosynthesis to enhance defence against phytophthora infestans in potato
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170774/
https://www.ncbi.nlm.nih.gov/pubmed/32077242
http://dx.doi.org/10.1111/mpp.12919
work_keys_str_mv AT liuhaifeng copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT xuexiaojing copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT yuyue copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT xumingming copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT luchongchong copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT mengxuanlin copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT zhangbaogang copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT dingxinhua copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato
AT chuzhaohui copperionssuppressabscisicacidbiosynthesistoenhancedefenceagainstphytophthorainfestansinpotato