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A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6

BACKGROUND: The non‐small cell lung cancer (NSCLC) is a common malignancy worldwide. Numerous reports have shown the critical role of long non‐coding RNAs (lncRNAs) in NSCLC. However, the role of a novel lncRNA named LNBC3 is still unknown. METHODS: By lncRNA profiling, novel lncRNAs related to NSCL...

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Autores principales: Shen, Jian, Ma, Jun, Li, Jianghong, Wang, Xin, Wang, Yi, Ma, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171294/
https://www.ncbi.nlm.nih.gov/pubmed/31743519
http://dx.doi.org/10.1002/jcla.23122
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author Shen, Jian
Ma, Jun
Li, Jianghong
Wang, Xin
Wang, Yi
Ma, Jie
author_facet Shen, Jian
Ma, Jun
Li, Jianghong
Wang, Xin
Wang, Yi
Ma, Jie
author_sort Shen, Jian
collection PubMed
description BACKGROUND: The non‐small cell lung cancer (NSCLC) is a common malignancy worldwide. Numerous reports have shown the critical role of long non‐coding RNAs (lncRNAs) in NSCLC. However, the role of a novel lncRNA named LNBC3 is still unknown. METHODS: By lncRNA profiling, novel lncRNAs related to NSCLC were identified. LNBC3 expression was quantified by qRT‐PCR. Migration and viability assays were performed to evaluate the function of LNBC3 in vitro. In vivo xenograft model was conducted to determine the oncogenic functions of LNBC3. RNA immunoprecipitation (RIP) followed by mass spectrometry (MS) was utilized to identify BCL6 as LNBC3 binding target. RESULTS: LNBC3 is markedly overexpressed in tumor tissues and NSCLC cell lines. Higher LNBC3 levels correlated with advanced TNM stages, larger tumor size, and metastasis. LNBC3 promoted NSCLC migration and viability. The in vivo experiments demonstrated that xenograft tumor growth and proliferation were facilitated with increasing LNBC3 levels. The antisense oligonucleotides (ASOs) targeting LNBC3 substantially inhibited lung cancer progression. Mechanistic studies showed that LNBC3 could interact with BCL6 leading to BCL6 stabilization through reduced proteasomal degradation. CONCLUSIONS: Collectively, our data have identified a novel lncRNA LNBC3 in NSCLC progression. The LNBC3‐BCL6 axis might be a potential target for pharmaceutical intervention.
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spelling pubmed-71712942020-04-21 A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6 Shen, Jian Ma, Jun Li, Jianghong Wang, Xin Wang, Yi Ma, Jie J Clin Lab Anal Research Articles BACKGROUND: The non‐small cell lung cancer (NSCLC) is a common malignancy worldwide. Numerous reports have shown the critical role of long non‐coding RNAs (lncRNAs) in NSCLC. However, the role of a novel lncRNA named LNBC3 is still unknown. METHODS: By lncRNA profiling, novel lncRNAs related to NSCLC were identified. LNBC3 expression was quantified by qRT‐PCR. Migration and viability assays were performed to evaluate the function of LNBC3 in vitro. In vivo xenograft model was conducted to determine the oncogenic functions of LNBC3. RNA immunoprecipitation (RIP) followed by mass spectrometry (MS) was utilized to identify BCL6 as LNBC3 binding target. RESULTS: LNBC3 is markedly overexpressed in tumor tissues and NSCLC cell lines. Higher LNBC3 levels correlated with advanced TNM stages, larger tumor size, and metastasis. LNBC3 promoted NSCLC migration and viability. The in vivo experiments demonstrated that xenograft tumor growth and proliferation were facilitated with increasing LNBC3 levels. The antisense oligonucleotides (ASOs) targeting LNBC3 substantially inhibited lung cancer progression. Mechanistic studies showed that LNBC3 could interact with BCL6 leading to BCL6 stabilization through reduced proteasomal degradation. CONCLUSIONS: Collectively, our data have identified a novel lncRNA LNBC3 in NSCLC progression. The LNBC3‐BCL6 axis might be a potential target for pharmaceutical intervention. John Wiley and Sons Inc. 2019-11-19 /pmc/articles/PMC7171294/ /pubmed/31743519 http://dx.doi.org/10.1002/jcla.23122 Text en © 2019 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Shen, Jian
Ma, Jun
Li, Jianghong
Wang, Xin
Wang, Yi
Ma, Jie
A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6
title A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6
title_full A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6
title_fullStr A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6
title_full_unstemmed A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6
title_short A long non‐coding RNA LNBC3 facilitates non‐small cell lung cancer progression by stabilizing BCL6
title_sort long non‐coding rna lnbc3 facilitates non‐small cell lung cancer progression by stabilizing bcl6
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171294/
https://www.ncbi.nlm.nih.gov/pubmed/31743519
http://dx.doi.org/10.1002/jcla.23122
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