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Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3

Hepatocellular carcinoma (HCC) is a main cause of cancer‐related deaths globally. Long non‐coding RNAs (lncRNAs) play important roles in diverse cancers. Our previous microarray‐based lncRNA profiling showed that LINC00467 was highly expressed in HCC. Here, we further explored the expression, role a...

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Autores principales: Wang, Haihao, Guo, Qiannan, Nampoukime, Kan‐Paatib Barnabo, Yang, Peiwen, Ma, Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171408/
https://www.ncbi.nlm.nih.gov/pubmed/32125766
http://dx.doi.org/10.1111/jcmm.14942
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author Wang, Haihao
Guo, Qiannan
Nampoukime, Kan‐Paatib Barnabo
Yang, Peiwen
Ma, Ke
author_facet Wang, Haihao
Guo, Qiannan
Nampoukime, Kan‐Paatib Barnabo
Yang, Peiwen
Ma, Ke
author_sort Wang, Haihao
collection PubMed
description Hepatocellular carcinoma (HCC) is a main cause of cancer‐related deaths globally. Long non‐coding RNAs (lncRNAs) play important roles in diverse cancers. Our previous microarray‐based lncRNA profiling showed that LINC00467 was highly expressed in HCC. Here, we further explored the expression, role and functional mechanism of lncRNA LINC00467 in HCC. Our findings revealed that LINC00467 was up‐regulated in HCC tissues and HCC cell lines. Increased expression of LINC00467 was positively associated with tumour size and vascular invasion. In vitro functional experiments revealed that LINC00467 accelerated HCC cell proliferation, cell cycle progression and migration and reduced HCC cell apoptosis. In vivo functional assays revealed that LINC00467 drove HCC xenograft growth and HCC cell proliferation and repressed HCC cell apoptosis in vivo. Moreover, LINC00467 inhibited NR4A3 post‐transcriptionally via interacting with NR4A3 mRNA to form double‐stranded RNA, which was further degraded by Dicer. The expression of NR4A3 was inversely associated with LINC00467 in HCC tissues. Functional rescue assays found that restore of NR4A3 expression blocked the oncogenic roles of LINC00467 in HCC. Taken together, our results demonstrated that lncRNA LINC00467 was a novel highly expressed and oncogenic lncRNA in HCC via inhibiting NR4A3. Targeting LINC00467 or enhancing NR4A3 may be potential therapeutic strategies against HCC.
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spelling pubmed-71714082020-04-21 Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3 Wang, Haihao Guo, Qiannan Nampoukime, Kan‐Paatib Barnabo Yang, Peiwen Ma, Ke J Cell Mol Med Original Articles Hepatocellular carcinoma (HCC) is a main cause of cancer‐related deaths globally. Long non‐coding RNAs (lncRNAs) play important roles in diverse cancers. Our previous microarray‐based lncRNA profiling showed that LINC00467 was highly expressed in HCC. Here, we further explored the expression, role and functional mechanism of lncRNA LINC00467 in HCC. Our findings revealed that LINC00467 was up‐regulated in HCC tissues and HCC cell lines. Increased expression of LINC00467 was positively associated with tumour size and vascular invasion. In vitro functional experiments revealed that LINC00467 accelerated HCC cell proliferation, cell cycle progression and migration and reduced HCC cell apoptosis. In vivo functional assays revealed that LINC00467 drove HCC xenograft growth and HCC cell proliferation and repressed HCC cell apoptosis in vivo. Moreover, LINC00467 inhibited NR4A3 post‐transcriptionally via interacting with NR4A3 mRNA to form double‐stranded RNA, which was further degraded by Dicer. The expression of NR4A3 was inversely associated with LINC00467 in HCC tissues. Functional rescue assays found that restore of NR4A3 expression blocked the oncogenic roles of LINC00467 in HCC. Taken together, our results demonstrated that lncRNA LINC00467 was a novel highly expressed and oncogenic lncRNA in HCC via inhibiting NR4A3. Targeting LINC00467 or enhancing NR4A3 may be potential therapeutic strategies against HCC. John Wiley and Sons Inc. 2020-03-03 2020-04 /pmc/articles/PMC7171408/ /pubmed/32125766 http://dx.doi.org/10.1111/jcmm.14942 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Haihao
Guo, Qiannan
Nampoukime, Kan‐Paatib Barnabo
Yang, Peiwen
Ma, Ke
Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3
title Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3
title_full Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3
title_fullStr Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3
title_full_unstemmed Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3
title_short Long non‐coding RNA LINC00467 drives hepatocellular carcinoma progression via inhibiting NR4A3
title_sort long non‐coding rna linc00467 drives hepatocellular carcinoma progression via inhibiting nr4a3
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171408/
https://www.ncbi.nlm.nih.gov/pubmed/32125766
http://dx.doi.org/10.1111/jcmm.14942
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