Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples

In this study, we describe a duplex real-time PCR assay for the simultaneous detection of KIPyV and WUPyV polyomaviruses based on TaqMan probes. This assay detected 500 copies/mL both for KIPyV and WUPyV in 100% of tested positive samples. We assessed this technique on 482 nasopharyngeal aspirate sp...

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Detalles Bibliográficos
Autores principales: Ligozzi, Marco, Galia, Liliana, Carelli, Maria, Piccaluga, Pier Paolo, Diani, Erica, Gibellini, Davide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172048/
https://www.ncbi.nlm.nih.gov/pubmed/29883628
http://dx.doi.org/10.1016/j.mcp.2018.06.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172048/
https://www.ncbi.nlm.nih.gov/pubmed/29883628
http://dx.doi.org/10.1016/j.mcp.2018.06.001

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