A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus

A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was construc...

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Autores principales: Nagesha, H.S, Wang, L.-F, Shiell, B, Beddome, G, White, J.R, Irving, R.A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2001
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172176/
https://www.ncbi.nlm.nih.gov/pubmed/11164502
http://dx.doi.org/10.1016/S0166-0934(00)00266-4
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author Nagesha, H.S
Wang, L.-F
Shiell, B
Beddome, G
White, J.R
Irving, R.A
author_facet Nagesha, H.S
Wang, L.-F
Shiell, B
Beddome, G
White, J.R
Irving, R.A
author_sort Nagesha, H.S
collection PubMed
description A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was constructed in phage vector. Two scFv clones that were selected showed specific reactivity with conformational epitope VP7. The N-terminal 22 amino acid residues of 20E9 light chain were identical to that deduced from scFv DNA sequence. An in-frame TAG stop codon was found in the coding sequence and its potential role in regulating the expression and stability of scFv in phage is discussed.
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spelling pubmed-71721762020-04-22 A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus Nagesha, H.S Wang, L.-F Shiell, B Beddome, G White, J.R Irving, R.A J Virol Methods Brief Report A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was constructed in phage vector. Two scFv clones that were selected showed specific reactivity with conformational epitope VP7. The N-terminal 22 amino acid residues of 20E9 light chain were identical to that deduced from scFv DNA sequence. An in-frame TAG stop codon was found in the coding sequence and its potential role in regulating the expression and stability of scFv in phage is discussed. Elsevier Science B.V. 2001-02 2001-01-26 /pmc/articles/PMC7172176/ /pubmed/11164502 http://dx.doi.org/10.1016/S0166-0934(00)00266-4 Text en Copyright © 2001 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Brief Report
Nagesha, H.S
Wang, L.-F
Shiell, B
Beddome, G
White, J.R
Irving, R.A
A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
title A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
title_full A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
title_fullStr A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
title_full_unstemmed A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
title_short A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
title_sort single chain fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172176/
https://www.ncbi.nlm.nih.gov/pubmed/11164502
http://dx.doi.org/10.1016/S0166-0934(00)00266-4
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