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Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants

BACKGROUND: Human respiratory syncytial virus (hRSV) detection in nasopharyngeal aspirates (NPAs) from infants with acute respiratory tract infection (ARTI) does not prove the hRSV etiology of the current ARTI episode. HRSV RNA quantification may help in affording this issue. OBJECTIVES: hRSV was de...

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Autores principales: Campanini, Giulia, Percivalle, Elena, Baldanti, Fausto, Rovida, Francesca, Bertaina, Alice, Marchi, Antonietta, Stronati, Mauro, Gerna, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172186/
https://www.ncbi.nlm.nih.gov/pubmed/17452001
http://dx.doi.org/10.1016/j.jcv.2007.03.009
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author Campanini, Giulia
Percivalle, Elena
Baldanti, Fausto
Rovida, Francesca
Bertaina, Alice
Marchi, Antonietta
Stronati, Mauro
Gerna, Giuseppe
author_facet Campanini, Giulia
Percivalle, Elena
Baldanti, Fausto
Rovida, Francesca
Bertaina, Alice
Marchi, Antonietta
Stronati, Mauro
Gerna, Giuseppe
author_sort Campanini, Giulia
collection PubMed
description BACKGROUND: Human respiratory syncytial virus (hRSV) detection in nasopharyngeal aspirates (NPAs) from infants with acute respiratory tract infection (ARTI) does not prove the hRSV etiology of the current ARTI episode. HRSV RNA quantification may help in affording this issue. OBJECTIVES: hRSV was detected by quantitative reverse transcription-PCR in NPAs taken upon admission to hospital and, whenever possible, at discharge and subsequent medical visits. STUDY DESIGN: Prospective study, including 63 infants affected by either hRSV upper or lower ARTI. RESULTS: Based on the kinetics of viral load, hRSV etiology was identified in 25 infants in whom hRSV load dropped from 2.5 × 10(6) upon admission (presence of respiratory symptoms) to 7.5 × 10(2) RNA copies/ml NPA upon discharge (absence of symptoms) after a median time of 5 days, and in 19 infants, in whom hRSV load was determined at admission only, in association with clinical symptoms (2.4 × 10(6) copies/ml). Furthermore, low levels of hRSV RNA (<1 × 10(5) copies/ml NPA) identified 14 patients with non-hRSV ARTI. Finally, in 14 infants with hRSV coinfections or sequential infections, hRSV quantification defined the hRSV role in the current ARTI episode. CONCLUSIONS: hRSV RNA quantification is critical in defining the hRSV role in respiratory infections.
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spelling pubmed-71721862020-04-22 Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants Campanini, Giulia Percivalle, Elena Baldanti, Fausto Rovida, Francesca Bertaina, Alice Marchi, Antonietta Stronati, Mauro Gerna, Giuseppe J Clin Virol Article BACKGROUND: Human respiratory syncytial virus (hRSV) detection in nasopharyngeal aspirates (NPAs) from infants with acute respiratory tract infection (ARTI) does not prove the hRSV etiology of the current ARTI episode. HRSV RNA quantification may help in affording this issue. OBJECTIVES: hRSV was detected by quantitative reverse transcription-PCR in NPAs taken upon admission to hospital and, whenever possible, at discharge and subsequent medical visits. STUDY DESIGN: Prospective study, including 63 infants affected by either hRSV upper or lower ARTI. RESULTS: Based on the kinetics of viral load, hRSV etiology was identified in 25 infants in whom hRSV load dropped from 2.5 × 10(6) upon admission (presence of respiratory symptoms) to 7.5 × 10(2) RNA copies/ml NPA upon discharge (absence of symptoms) after a median time of 5 days, and in 19 infants, in whom hRSV load was determined at admission only, in association with clinical symptoms (2.4 × 10(6) copies/ml). Furthermore, low levels of hRSV RNA (<1 × 10(5) copies/ml NPA) identified 14 patients with non-hRSV ARTI. Finally, in 14 infants with hRSV coinfections or sequential infections, hRSV quantification defined the hRSV role in the current ARTI episode. CONCLUSIONS: hRSV RNA quantification is critical in defining the hRSV role in respiratory infections. Elsevier B.V. 2007-06 2007-04-23 /pmc/articles/PMC7172186/ /pubmed/17452001 http://dx.doi.org/10.1016/j.jcv.2007.03.009 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Campanini, Giulia
Percivalle, Elena
Baldanti, Fausto
Rovida, Francesca
Bertaina, Alice
Marchi, Antonietta
Stronati, Mauro
Gerna, Giuseppe
Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
title Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
title_full Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
title_fullStr Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
title_full_unstemmed Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
title_short Human respiratory syncytial virus (hRSV) RNA quantification in nasopharyngeal secretions identifies the hRSV etiologic role in acute respiratory tract infections of hospitalized infants
title_sort human respiratory syncytial virus (hrsv) rna quantification in nasopharyngeal secretions identifies the hrsv etiologic role in acute respiratory tract infections of hospitalized infants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172186/
https://www.ncbi.nlm.nih.gov/pubmed/17452001
http://dx.doi.org/10.1016/j.jcv.2007.03.009
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