Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()

The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional...

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Autores principales: Bastien, Nathalie, Normand, Susan, Taylor, Tracy, Ward, Diane, Peret, Teresa C.T, Boivin, Guy, Anderson, Larry J, Li, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2003
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172423/
https://www.ncbi.nlm.nih.gov/pubmed/12727342
http://dx.doi.org/10.1016/S0168-1702(03)00065-0
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author Bastien, Nathalie
Normand, Susan
Taylor, Tracy
Ward, Diane
Peret, Teresa C.T
Boivin, Guy
Anderson, Larry J
Li, Yan
author_facet Bastien, Nathalie
Normand, Susan
Taylor, Tracy
Ward, Diane
Peret, Teresa C.T
Boivin, Guy
Anderson, Larry J
Li, Yan
author_sort Bastien, Nathalie
collection PubMed
description The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional sequence variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F genes of the different isolates revealed that all three genes were well conserved with 94.1–97.6% identity between the two distinct clusters The P gene showed more diversity with 81.6–85.7% amino acid identity for isolates between the two clusters, and 94.6–100% for isolates within the same cluster.
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spelling pubmed-71724232020-04-22 Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains() Bastien, Nathalie Normand, Susan Taylor, Tracy Ward, Diane Peret, Teresa C.T Boivin, Guy Anderson, Larry J Li, Yan Virus Res Article The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional sequence variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F genes of the different isolates revealed that all three genes were well conserved with 94.1–97.6% identity between the two distinct clusters The P gene showed more diversity with 81.6–85.7% amino acid identity for isolates between the two clusters, and 94.6–100% for isolates within the same cluster. Elsevier Science B.V. 2003-05 2003-04-03 /pmc/articles/PMC7172423/ /pubmed/12727342 http://dx.doi.org/10.1016/S0168-1702(03)00065-0 Text en Copyright © 2003 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Bastien, Nathalie
Normand, Susan
Taylor, Tracy
Ward, Diane
Peret, Teresa C.T
Boivin, Guy
Anderson, Larry J
Li, Yan
Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()
title Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()
title_full Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()
title_fullStr Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()
title_full_unstemmed Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()
title_short Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains()
title_sort sequence analysis of the n, p, m and f genes of canadian human metapneumovirus strains()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172423/
https://www.ncbi.nlm.nih.gov/pubmed/12727342
http://dx.doi.org/10.1016/S0168-1702(03)00065-0
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