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Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue

Multiple sclerosis (MS) is an important demyelinating disease of the central nervous system, the aetiology of which is thought to have a possible viral component. In this study we investigated the possible involvement in MS of two herpes viruses: the neurotropic human herpesvirus 7 (HHV-7) and the r...

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Autores principales: Opsahl, Margaret L., Kennedy, Peter G.E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172428/
https://www.ncbi.nlm.nih.gov/pubmed/16242723
http://dx.doi.org/10.1016/j.jns.2005.08.014
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author Opsahl, Margaret L.
Kennedy, Peter G.E.
author_facet Opsahl, Margaret L.
Kennedy, Peter G.E.
author_sort Opsahl, Margaret L.
collection PubMed
description Multiple sclerosis (MS) is an important demyelinating disease of the central nervous system, the aetiology of which is thought to have a possible viral component. In this study we investigated the possible involvement in MS of two herpes viruses: the neurotropic human herpesvirus 7 (HHV-7) and the related human herpesvirus 8 (HHV-8). Utilising fluorescent in situ hybridisation (FISH) techniques, we examined human post mortem tissues for the presence of immediate early and late viral gene or protein expression in MS patient normal appearing white matter (NAWM), lesional tissue and normal control brain samples. HHV-7 and/or HHV-8 mRNA or protein was detected in some individuals in all three sample categories and was restricted to oligodendrocytes, as determined by double mRNA FISH analysis or immuno fluorescence (IF). No samples showed evidence of viral mRNA when subjected to RT-PCR on extracted ribonucleic acid. We therefore conclude that there is little evidence in our particular sample cohort to suggest involvement of either HHV-7 or HHV-8 in MS.
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spelling pubmed-71724282020-04-22 Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue Opsahl, Margaret L. Kennedy, Peter G.E. J Neurol Sci Article Multiple sclerosis (MS) is an important demyelinating disease of the central nervous system, the aetiology of which is thought to have a possible viral component. In this study we investigated the possible involvement in MS of two herpes viruses: the neurotropic human herpesvirus 7 (HHV-7) and the related human herpesvirus 8 (HHV-8). Utilising fluorescent in situ hybridisation (FISH) techniques, we examined human post mortem tissues for the presence of immediate early and late viral gene or protein expression in MS patient normal appearing white matter (NAWM), lesional tissue and normal control brain samples. HHV-7 and/or HHV-8 mRNA or protein was detected in some individuals in all three sample categories and was restricted to oligodendrocytes, as determined by double mRNA FISH analysis or immuno fluorescence (IF). No samples showed evidence of viral mRNA when subjected to RT-PCR on extracted ribonucleic acid. We therefore conclude that there is little evidence in our particular sample cohort to suggest involvement of either HHV-7 or HHV-8 in MS. Elsevier B.V. 2006-01-15 2005-10-20 /pmc/articles/PMC7172428/ /pubmed/16242723 http://dx.doi.org/10.1016/j.jns.2005.08.014 Text en Copyright © 2005 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Opsahl, Margaret L.
Kennedy, Peter G.E.
Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
title Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
title_full Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
title_fullStr Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
title_full_unstemmed Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
title_short Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
title_sort investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172428/
https://www.ncbi.nlm.nih.gov/pubmed/16242723
http://dx.doi.org/10.1016/j.jns.2005.08.014
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