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Universal virus detection by degenerate-oligonucleotide primed polymerase chain reaction of purified viral nucleic acids
This study describes a novel non-specific universal virus detection method that permits molecular detection of viruses in biological materials containing mixtures of cells and viruses. Samples are subjected to nuclease digestion and ultracentrifugation to separate encapsidated viral nucleic acids fr...
Autores principales: | Nanda, Santosh, Jayan, Geetha, Voulgaropoulou, Frosso, Sierra-Honigmann, Ana Maria, Uhlenhaut, Christine, McWatters, Bernard J.P., Patel, Amita, Krause, Philip R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172433/ https://www.ncbi.nlm.nih.gov/pubmed/18601953 http://dx.doi.org/10.1016/j.jviromet.2008.06.007 |
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