Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA

The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are...

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Detalles Bibliográficos
Autores principales: Fujita, Motomichi, Adachi, Koji, Nagasawa, Michiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172543/
https://www.ncbi.nlm.nih.gov/pubmed/30352199
http://dx.doi.org/10.1016/j.ab.2018.10.021
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author Fujita, Motomichi
Adachi, Koji
Nagasawa, Michiaki
author_facet Fujita, Motomichi
Adachi, Koji
Nagasawa, Michiaki
author_sort Fujita, Motomichi
collection PubMed
description The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are required. Here, we describe a novel method for measurement of double-stranded RNA using a homogeneous time-resolved fluorescence assay. This method allowed detection of human rhinovirus (HRV), enterovirus, coxsackievirus, and murine norovirus. Furthermore, this method detected antiviral activity of a HRV 3C protease inhibitor. The assay may be useful for discovery of antiviral agents against (+) ssRNA viruses.
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spelling pubmed-71725432020-04-22 Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA Fujita, Motomichi Adachi, Koji Nagasawa, Michiaki Anal Biochem Article The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are required. Here, we describe a novel method for measurement of double-stranded RNA using a homogeneous time-resolved fluorescence assay. This method allowed detection of human rhinovirus (HRV), enterovirus, coxsackievirus, and murine norovirus. Furthermore, this method detected antiviral activity of a HRV 3C protease inhibitor. The assay may be useful for discovery of antiviral agents against (+) ssRNA viruses. Elsevier Inc. 2019-02-01 2018-10-21 /pmc/articles/PMC7172543/ /pubmed/30352199 http://dx.doi.org/10.1016/j.ab.2018.10.021 Text en © 2018 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Fujita, Motomichi
Adachi, Koji
Nagasawa, Michiaki
Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
title Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
title_full Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
title_fullStr Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
title_full_unstemmed Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
title_short Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
title_sort development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172543/
https://www.ncbi.nlm.nih.gov/pubmed/30352199
http://dx.doi.org/10.1016/j.ab.2018.10.021
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