Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide

Rubella virus was purified by chromatography on Sepharose 2B after concentration by ultrafiltration on hollow fibers and hydroextraction with PEG 20,000. Yields of 40% infectivity and 70% hemagglutinating activity were routinely obtained. Chromatographie purification was compared to ultracentrifugat...

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Detalles Bibliográficos
Autores principales: Trudel, M., Marchessault, F., Payment, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1981
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172648/
https://www.ncbi.nlm.nih.gov/pubmed/7276127
http://dx.doi.org/10.1016/0166-0934(81)90032-X
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author Trudel, M.
Marchessault, F.
Payment, P.
author_facet Trudel, M.
Marchessault, F.
Payment, P.
author_sort Trudel, M.
collection PubMed
description Rubella virus was purified by chromatography on Sepharose 2B after concentration by ultrafiltration on hollow fibers and hydroextraction with PEG 20,000. Yields of 40% infectivity and 70% hemagglutinating activity were routinely obtained. Chromatographie purification was compared to ultracentrifugation in sucrose, metrizamide and sodium metrizoate. Yields were lower in sucrose and metrizamide, while sodium metrizoate reduced the infectivity of the virus below detectable levels. These results demonstrate the advantage of Sepharose 2B for the purification of infectious rubella virus.
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spelling pubmed-71726482020-04-22 Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide Trudel, M. Marchessault, F. Payment, P. J Virol Methods Article Rubella virus was purified by chromatography on Sepharose 2B after concentration by ultrafiltration on hollow fibers and hydroextraction with PEG 20,000. Yields of 40% infectivity and 70% hemagglutinating activity were routinely obtained. Chromatographie purification was compared to ultracentrifugation in sucrose, metrizamide and sodium metrizoate. Yields were lower in sucrose and metrizamide, while sodium metrizoate reduced the infectivity of the virus below detectable levels. These results demonstrate the advantage of Sepharose 2B for the purification of infectious rubella virus. Published by Elsevier B.V. 1981-02 2002-11-12 /pmc/articles/PMC7172648/ /pubmed/7276127 http://dx.doi.org/10.1016/0166-0934(81)90032-X Text en Copyright © 1981 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Trudel, M.
Marchessault, F.
Payment, P.
Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
title Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
title_full Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
title_fullStr Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
title_full_unstemmed Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
title_short Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
title_sort purification of infectious rubella virus by gel filtration on sepharose 2b compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172648/
https://www.ncbi.nlm.nih.gov/pubmed/7276127
http://dx.doi.org/10.1016/0166-0934(81)90032-X
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