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Multiple novel and prevalent astroviruses in pigs()

Knowledge of porcine astrovirus diversity and epidemiology remains limited. We used a broad range PCR approach to investigate the presence and diversity of astroviruses in healthy pigs of different ages on 20 farms and in 3 slaughterhouses situated in the province of Quebec, Canada between 2005 and...

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Autores principales: Luo, Zhiyao, Roi, Stéphanie, Dastor, Margaux, Gallice, Estelle, Laurin, Marc-André, L’Homme, Yvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172684/
https://www.ncbi.nlm.nih.gov/pubmed/21159453
http://dx.doi.org/10.1016/j.vetmic.2010.11.026
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author Luo, Zhiyao
Roi, Stéphanie
Dastor, Margaux
Gallice, Estelle
Laurin, Marc-André
L’Homme, Yvan
author_facet Luo, Zhiyao
Roi, Stéphanie
Dastor, Margaux
Gallice, Estelle
Laurin, Marc-André
L’Homme, Yvan
author_sort Luo, Zhiyao
collection PubMed
description Knowledge of porcine astrovirus diversity and epidemiology remains limited. We used a broad range PCR approach to investigate the presence and diversity of astroviruses in healthy pigs of different ages on 20 farms and in 3 slaughterhouses situated in the province of Quebec, Canada between 2005 and 2007. Our study unexpectedly revealed remarkable levels of genetic diversity and high prevalence of astroviruses in pigs of this province. Astroviruses were detected on every farm investigated and in all age groups of pigs, from suckling piglets to adults. In addition, we found that nearly 80% of healthy finisher pigs harbour astroviruses in their intestine at slaughter. Phylogenetic evidence based on partial polymerase and complete capsid sequences, suggests that porcine astroviruses do not form a monophyletic group but are rather found on separate branches across the mamastrovirus tree. In addition to type species strains, we found highly divergent strains that form two additional lineages, one of which falls outside existing taxonomic groups. The presence of diverse astroviruses in a majority of healthy pigs likely represents a continuous source of infection to piglets and possibly to other animal species including humans. Porcine astrovirus strains appeared phylogenetically related not only to prototypical human astroviruses, as was already known, but also to novel human strains recently discovered suggesting multiple cross species transmission events between these hosts and other animal species. Overall, the findings reported in this study suggest an active role of pigs in the evolution and ecology of the Astroviridae.
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spelling pubmed-71726842020-04-22 Multiple novel and prevalent astroviruses in pigs() Luo, Zhiyao Roi, Stéphanie Dastor, Margaux Gallice, Estelle Laurin, Marc-André L’Homme, Yvan Vet Microbiol Article Knowledge of porcine astrovirus diversity and epidemiology remains limited. We used a broad range PCR approach to investigate the presence and diversity of astroviruses in healthy pigs of different ages on 20 farms and in 3 slaughterhouses situated in the province of Quebec, Canada between 2005 and 2007. Our study unexpectedly revealed remarkable levels of genetic diversity and high prevalence of astroviruses in pigs of this province. Astroviruses were detected on every farm investigated and in all age groups of pigs, from suckling piglets to adults. In addition, we found that nearly 80% of healthy finisher pigs harbour astroviruses in their intestine at slaughter. Phylogenetic evidence based on partial polymerase and complete capsid sequences, suggests that porcine astroviruses do not form a monophyletic group but are rather found on separate branches across the mamastrovirus tree. In addition to type species strains, we found highly divergent strains that form two additional lineages, one of which falls outside existing taxonomic groups. The presence of diverse astroviruses in a majority of healthy pigs likely represents a continuous source of infection to piglets and possibly to other animal species including humans. Porcine astrovirus strains appeared phylogenetically related not only to prototypical human astroviruses, as was already known, but also to novel human strains recently discovered suggesting multiple cross species transmission events between these hosts and other animal species. Overall, the findings reported in this study suggest an active role of pigs in the evolution and ecology of the Astroviridae. Published by Elsevier B.V. 2011-05-05 2010-11-24 /pmc/articles/PMC7172684/ /pubmed/21159453 http://dx.doi.org/10.1016/j.vetmic.2010.11.026 Text en Crown copyright © 2010 Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Luo, Zhiyao
Roi, Stéphanie
Dastor, Margaux
Gallice, Estelle
Laurin, Marc-André
L’Homme, Yvan
Multiple novel and prevalent astroviruses in pigs()
title Multiple novel and prevalent astroviruses in pigs()
title_full Multiple novel and prevalent astroviruses in pigs()
title_fullStr Multiple novel and prevalent astroviruses in pigs()
title_full_unstemmed Multiple novel and prevalent astroviruses in pigs()
title_short Multiple novel and prevalent astroviruses in pigs()
title_sort multiple novel and prevalent astroviruses in pigs()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172684/
https://www.ncbi.nlm.nih.gov/pubmed/21159453
http://dx.doi.org/10.1016/j.vetmic.2010.11.026
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