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Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet

Next-generation sequencing (NGS) technologies are becoming increasingly accessible, leading to an expanded interest in the composition of the porcine enteric virome. In the present study, the fecal virome of a non-diarrheic Belgian piglet was determined. Although the virome of only a single piglet w...

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Autores principales: Theuns, Sebastiaan, Conceição-Neto, Nádia, Zeller, Mark, Heylen, Elisabeth, Roukaerts, Inge D.M., Desmarets, Lowiese M.B., Van Ranst, Marc, Nauwynck, Hans J., Matthijnssens, Jelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172746/
https://www.ncbi.nlm.nih.gov/pubmed/27184192
http://dx.doi.org/10.1016/j.meegid.2016.05.018
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author Theuns, Sebastiaan
Conceição-Neto, Nádia
Zeller, Mark
Heylen, Elisabeth
Roukaerts, Inge D.M.
Desmarets, Lowiese M.B.
Van Ranst, Marc
Nauwynck, Hans J.
Matthijnssens, Jelle
author_facet Theuns, Sebastiaan
Conceição-Neto, Nádia
Zeller, Mark
Heylen, Elisabeth
Roukaerts, Inge D.M.
Desmarets, Lowiese M.B.
Van Ranst, Marc
Nauwynck, Hans J.
Matthijnssens, Jelle
author_sort Theuns, Sebastiaan
collection PubMed
description Next-generation sequencing (NGS) technologies are becoming increasingly accessible, leading to an expanded interest in the composition of the porcine enteric virome. In the present study, the fecal virome of a non-diarrheic Belgian piglet was determined. Although the virome of only a single piglet was analyzed, some interesting data were obtained, including the second complete genome of a pig group C rotavirus (RVC). This Belgian strain was only distantly related to the only other completely characterized pig RVC strain, Cowden. Its relatedness to RVC strains from other host species was also analyzed and the porcine strain found in our study was only distantly related to RVCs detected in humans and cows. The gene encoding the outer capsid protein VP7 belonged to the rare porcine G3 genotype, which might be serologically distinct from most other pig RVC strains. A putative novel RVC VP6 genotype was identified as well. A group A rotavirus strain also present in this fecal sample contained the rare pig genotype combination G11P[27], but was only partially characterized. Typical pig RVA genotypes I5, A8, and T7 were found for the viral proteins VP6, NSP1, and NSP3, respectively. Interestingly, the fecal virome of the piglet also contained an astrovirus and an enterovirus, of which the complete genomes were characterized. Results of the current study indicate that many viruses may be present simultaneously in fecal samples of non-diarrheic piglets. In this study, these viruses could not be directly associated with any disease, but still they might have had a potential subclinical impact on pig growth performance. The fast evolution of NGS will be a powerful tool for future diagnostics in veterinary practice. Its application will certainly lead to better insights into the relevance of many (sub)clinical enteric viral infections, that may have remained unnoticed using traditional diagnostic techniques. This will stimulate the development of new and durable prophylactic measures to improve pig health and production.
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spelling pubmed-71727462020-04-22 Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet Theuns, Sebastiaan Conceição-Neto, Nádia Zeller, Mark Heylen, Elisabeth Roukaerts, Inge D.M. Desmarets, Lowiese M.B. Van Ranst, Marc Nauwynck, Hans J. Matthijnssens, Jelle Infect Genet Evol Research Paper Next-generation sequencing (NGS) technologies are becoming increasingly accessible, leading to an expanded interest in the composition of the porcine enteric virome. In the present study, the fecal virome of a non-diarrheic Belgian piglet was determined. Although the virome of only a single piglet was analyzed, some interesting data were obtained, including the second complete genome of a pig group C rotavirus (RVC). This Belgian strain was only distantly related to the only other completely characterized pig RVC strain, Cowden. Its relatedness to RVC strains from other host species was also analyzed and the porcine strain found in our study was only distantly related to RVCs detected in humans and cows. The gene encoding the outer capsid protein VP7 belonged to the rare porcine G3 genotype, which might be serologically distinct from most other pig RVC strains. A putative novel RVC VP6 genotype was identified as well. A group A rotavirus strain also present in this fecal sample contained the rare pig genotype combination G11P[27], but was only partially characterized. Typical pig RVA genotypes I5, A8, and T7 were found for the viral proteins VP6, NSP1, and NSP3, respectively. Interestingly, the fecal virome of the piglet also contained an astrovirus and an enterovirus, of which the complete genomes were characterized. Results of the current study indicate that many viruses may be present simultaneously in fecal samples of non-diarrheic piglets. In this study, these viruses could not be directly associated with any disease, but still they might have had a potential subclinical impact on pig growth performance. The fast evolution of NGS will be a powerful tool for future diagnostics in veterinary practice. Its application will certainly lead to better insights into the relevance of many (sub)clinical enteric viral infections, that may have remained unnoticed using traditional diagnostic techniques. This will stimulate the development of new and durable prophylactic measures to improve pig health and production. Elsevier B.V. 2016-09 2016-05-14 /pmc/articles/PMC7172746/ /pubmed/27184192 http://dx.doi.org/10.1016/j.meegid.2016.05.018 Text en © 2016 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Research Paper
Theuns, Sebastiaan
Conceição-Neto, Nádia
Zeller, Mark
Heylen, Elisabeth
Roukaerts, Inge D.M.
Desmarets, Lowiese M.B.
Van Ranst, Marc
Nauwynck, Hans J.
Matthijnssens, Jelle
Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet
title Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet
title_full Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet
title_fullStr Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet
title_full_unstemmed Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet
title_short Characterization of a genetically heterogeneous porcine rotavirus C, and other viruses present in the fecal virome of a non-diarrheic Belgian piglet
title_sort characterization of a genetically heterogeneous porcine rotavirus c, and other viruses present in the fecal virome of a non-diarrheic belgian piglet
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172746/
https://www.ncbi.nlm.nih.gov/pubmed/27184192
http://dx.doi.org/10.1016/j.meegid.2016.05.018
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