A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()

An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titr...

Descripción completa

Detalles Bibliográficos
Autores principales: Roberts, Peter C., Etchison, James R., Bond, Clifford W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1988
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172778/
https://www.ncbi.nlm.nih.gov/pubmed/2466364
http://dx.doi.org/10.1016/0378-1135(88)90088-0
_version_ 1783524323073458176
author Roberts, Peter C.
Etchison, James R.
Bond, Clifford W.
author_facet Roberts, Peter C.
Etchison, James R.
Bond, Clifford W.
author_sort Roberts, Peter C.
collection PubMed
description An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titre is derived from the number of uninfected wells, using the Poisson distribution. The assay is simple, fast and economical. Titres of cytopathic virus determined by the microtitration assay and standard plaque assay are equivalent.
format Online
Article
Text
id pubmed-7172778
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher Published by Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71727782020-04-22 A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus() Roberts, Peter C. Etchison, James R. Bond, Clifford W. Vet Microbiol Article An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titre is derived from the number of uninfected wells, using the Poisson distribution. The assay is simple, fast and economical. Titres of cytopathic virus determined by the microtitration assay and standard plaque assay are equivalent. Published by Elsevier B.V. 1988-12 2002-11-13 /pmc/articles/PMC7172778/ /pubmed/2466364 http://dx.doi.org/10.1016/0378-1135(88)90088-0 Text en Copyright © 1988 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Roberts, Peter C.
Etchison, James R.
Bond, Clifford W.
A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
title A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
title_full A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
title_fullStr A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
title_full_unstemmed A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
title_short A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
title_sort rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172778/
https://www.ncbi.nlm.nih.gov/pubmed/2466364
http://dx.doi.org/10.1016/0378-1135(88)90088-0
work_keys_str_mv AT robertspeterc arapidquantitativeassayfortitrationofbovinevirusdiarrhoeamucosaldiseasevirus
AT etchisonjamesr arapidquantitativeassayfortitrationofbovinevirusdiarrhoeamucosaldiseasevirus
AT bondcliffordw arapidquantitativeassayfortitrationofbovinevirusdiarrhoeamucosaldiseasevirus
AT robertspeterc rapidquantitativeassayfortitrationofbovinevirusdiarrhoeamucosaldiseasevirus
AT etchisonjamesr rapidquantitativeassayfortitrationofbovinevirusdiarrhoeamucosaldiseasevirus
AT bondcliffordw rapidquantitativeassayfortitrationofbovinevirusdiarrhoeamucosaldiseasevirus

Ejemplares similares