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Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody
Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immu...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172788/ https://www.ncbi.nlm.nih.gov/pubmed/28463713 http://dx.doi.org/10.1016/j.virol.2017.04.026 |
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author | Liu, Jianbo Shi, Hongyan Chen, Jianfei Zhang, Xin Ji, Zhaoyang Yuan, Jing Shi, Da Cao, Liyan Zhu, Xiangdong Dong, Hui Wang, Xiaobo Zhang, Jialin Feng, Li |
author_facet | Liu, Jianbo Shi, Hongyan Chen, Jianfei Zhang, Xin Ji, Zhaoyang Yuan, Jing Shi, Da Cao, Liyan Zhu, Xiangdong Dong, Hui Wang, Xiaobo Zhang, Jialin Feng, Li |
author_sort | Liu, Jianbo |
collection | PubMed |
description | Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutralized new variant genotype 2 PEDV strains (LNCT2, LNSY, and Hjms), but it did not neutralize a genotype 1 PEDV strain (CV777), in vitro. Results showed that the epitope recognized by the 1B9 mAb lies in the spike protein, and that it is a conformational epitope. These findings confirm that allelic differences in the PEDV S gene between the G1 and G2 genotype strains led to changes in the S protein and, thus, differences in its immunogenicity. |
format | Online Article Text |
id | pubmed-7172788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71727882020-04-22 Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody Liu, Jianbo Shi, Hongyan Chen, Jianfei Zhang, Xin Ji, Zhaoyang Yuan, Jing Shi, Da Cao, Liyan Zhu, Xiangdong Dong, Hui Wang, Xiaobo Zhang, Jialin Feng, Li Virology Article Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutralized new variant genotype 2 PEDV strains (LNCT2, LNSY, and Hjms), but it did not neutralize a genotype 1 PEDV strain (CV777), in vitro. Results showed that the epitope recognized by the 1B9 mAb lies in the spike protein, and that it is a conformational epitope. These findings confirm that allelic differences in the PEDV S gene between the G1 and G2 genotype strains led to changes in the S protein and, thus, differences in its immunogenicity. Published by Elsevier Inc. 2017-07 2017-04-29 /pmc/articles/PMC7172788/ /pubmed/28463713 http://dx.doi.org/10.1016/j.virol.2017.04.026 Text en © 2017 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Liu, Jianbo Shi, Hongyan Chen, Jianfei Zhang, Xin Ji, Zhaoyang Yuan, Jing Shi, Da Cao, Liyan Zhu, Xiangdong Dong, Hui Wang, Xiaobo Zhang, Jialin Feng, Li Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
title | Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
title_full | Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
title_fullStr | Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
title_full_unstemmed | Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
title_short | Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
title_sort | neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172788/ https://www.ncbi.nlm.nih.gov/pubmed/28463713 http://dx.doi.org/10.1016/j.virol.2017.04.026 |
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