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Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained

The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied. Genetic manipulation of full-length cDNA clones has become an especially important and widely used tool to study the biology,...

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Detalles Bibliográficos
Autores principales: Balasuriya, Udeni B.R., Zhang, Jianqiang, Go, Yun Young, MacLachlan, N. James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172799/
https://www.ncbi.nlm.nih.gov/pubmed/24913633
http://dx.doi.org/10.1016/j.virol.2014.04.029
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author Balasuriya, Udeni B.R.
Zhang, Jianqiang
Go, Yun Young
MacLachlan, N. James
author_facet Balasuriya, Udeni B.R.
Zhang, Jianqiang
Go, Yun Young
MacLachlan, N. James
author_sort Balasuriya, Udeni B.R.
collection PubMed
description The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied. Genetic manipulation of full-length cDNA clones has become an especially important and widely used tool to study the biology, pathogenesis, and virulence determinants of both positive and negative stranded RNA viruses. The first full-length infectious cDNA clone of equine arteritis virus (EAV) was developed in 1996 and was also the first full-length infectious cDNA clone constructed from a member of the order Nidovirales. This clone was extensively used to characterize the molecular biology of EAV and other Nidoviruses. The objective of this review is to summarize the characterization of the virulence (or attenuation) phenotype of the recombinant viruses derived from several infectious cDNA clones of EAV in horses, as well as their application for characterization of the molecular basis of viral neutralization, persistence, and cellular tropism.
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spelling pubmed-71727992020-04-22 Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained Balasuriya, Udeni B.R. Zhang, Jianqiang Go, Yun Young MacLachlan, N. James Virology Review The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied. Genetic manipulation of full-length cDNA clones has become an especially important and widely used tool to study the biology, pathogenesis, and virulence determinants of both positive and negative stranded RNA viruses. The first full-length infectious cDNA clone of equine arteritis virus (EAV) was developed in 1996 and was also the first full-length infectious cDNA clone constructed from a member of the order Nidovirales. This clone was extensively used to characterize the molecular biology of EAV and other Nidoviruses. The objective of this review is to summarize the characterization of the virulence (or attenuation) phenotype of the recombinant viruses derived from several infectious cDNA clones of EAV in horses, as well as their application for characterization of the molecular basis of viral neutralization, persistence, and cellular tropism. Elsevier Inc. 2014-08 2014-06-07 /pmc/articles/PMC7172799/ /pubmed/24913633 http://dx.doi.org/10.1016/j.virol.2014.04.029 Text en Copyright © 2014 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Review
Balasuriya, Udeni B.R.
Zhang, Jianqiang
Go, Yun Young
MacLachlan, N. James
Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
title Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
title_full Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
title_fullStr Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
title_full_unstemmed Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
title_short Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
title_sort experiences with infectious cdna clones of equine arteritis virus: lessons learned and insights gained
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172799/
https://www.ncbi.nlm.nih.gov/pubmed/24913633
http://dx.doi.org/10.1016/j.virol.2014.04.029
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