Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()

The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrap...

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Autores principales: Vey, Martin, Orlich, Michaela, Adler, Sabine, Klenk, Hans-Dieter, Rott, Rudolf, Garten, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172898/
https://www.ncbi.nlm.nih.gov/pubmed/1566583
http://dx.doi.org/10.1016/0042-6822(92)90775-K
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author Vey, Martin
Orlich, Michaela
Adler, Sabine
Klenk, Hans-Dieter
Rott, Rudolf
Garten, Wolfgang
author_facet Vey, Martin
Orlich, Michaela
Adler, Sabine
Klenk, Hans-Dieter
Rott, Rudolf
Garten, Wolfgang
author_sort Vey, Martin
collection PubMed
description The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrapeptide motif R-X-K/R-R that must be presented in the correct sequence position. Studies on plaque variants of influenza virus A/fowINictoria/75 (H7N7) showed that alteration of the consensus sequence resulted in a loss of pathogenicity for chickens.
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spelling pubmed-71728982020-04-22 Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R() Vey, Martin Orlich, Michaela Adler, Sabine Klenk, Hans-Dieter Rott, Rudolf Garten, Wolfgang Virology Article The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrapeptide motif R-X-K/R-R that must be presented in the correct sequence position. Studies on plaque variants of influenza virus A/fowINictoria/75 (H7N7) showed that alteration of the consensus sequence resulted in a loss of pathogenicity for chickens. Published by Elsevier Inc. 1992-05 2004-02-10 /pmc/articles/PMC7172898/ /pubmed/1566583 http://dx.doi.org/10.1016/0042-6822(92)90775-K Text en Copyright © 1992 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Vey, Martin
Orlich, Michaela
Adler, Sabine
Klenk, Hans-Dieter
Rott, Rudolf
Garten, Wolfgang
Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()
title Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()
title_full Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()
title_fullStr Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()
title_full_unstemmed Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()
title_short Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R()
title_sort hemagglutinin activation of pathogenic avian influenza viruses of serotype h7 requires the protease recognition motif r-x-k/r-r()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172898/
https://www.ncbi.nlm.nih.gov/pubmed/1566583
http://dx.doi.org/10.1016/0042-6822(92)90775-K
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