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Genome characterization of feline morbillivirus from Italy
Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next genera...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172958/ https://www.ncbi.nlm.nih.gov/pubmed/27155238 http://dx.doi.org/10.1016/j.jviromet.2016.05.002 |
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author | Marcacci, Maurilia De Luca, Eliana Zaccaria, Guendalina Di Tommaso, Morena Mangone, Iolanda Aste, Giovanni Savini, Giovanni Boari, Andrea Lorusso, Alessio |
author_facet | Marcacci, Maurilia De Luca, Eliana Zaccaria, Guendalina Di Tommaso, Morena Mangone, Iolanda Aste, Giovanni Savini, Giovanni Boari, Andrea Lorusso, Alessio |
author_sort | Marcacci, Maurilia |
collection | PubMed |
description | Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next generation sequencing (NGS) starting from RNA purified from the infected urine sample. The existence in Germany and Turkey of FeMVs from cats divergent from Piuma/2015, suggests the presence of FeMV heterogeneity in Europe as it has been described previously in Japan and China. |
format | Online Article Text |
id | pubmed-7172958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71729582020-04-22 Genome characterization of feline morbillivirus from Italy Marcacci, Maurilia De Luca, Eliana Zaccaria, Guendalina Di Tommaso, Morena Mangone, Iolanda Aste, Giovanni Savini, Giovanni Boari, Andrea Lorusso, Alessio J Virol Methods Article Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next generation sequencing (NGS) starting from RNA purified from the infected urine sample. The existence in Germany and Turkey of FeMVs from cats divergent from Piuma/2015, suggests the presence of FeMV heterogeneity in Europe as it has been described previously in Japan and China. Elsevier B.V. 2016-08 2016-05-04 /pmc/articles/PMC7172958/ /pubmed/27155238 http://dx.doi.org/10.1016/j.jviromet.2016.05.002 Text en © 2016 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Marcacci, Maurilia De Luca, Eliana Zaccaria, Guendalina Di Tommaso, Morena Mangone, Iolanda Aste, Giovanni Savini, Giovanni Boari, Andrea Lorusso, Alessio Genome characterization of feline morbillivirus from Italy |
title | Genome characterization of feline morbillivirus from Italy |
title_full | Genome characterization of feline morbillivirus from Italy |
title_fullStr | Genome characterization of feline morbillivirus from Italy |
title_full_unstemmed | Genome characterization of feline morbillivirus from Italy |
title_short | Genome characterization of feline morbillivirus from Italy |
title_sort | genome characterization of feline morbillivirus from italy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172958/ https://www.ncbi.nlm.nih.gov/pubmed/27155238 http://dx.doi.org/10.1016/j.jviromet.2016.05.002 |
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