The actin cytoskeleton is important for rotavirus internalization and RNA genome replication

Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dyna...

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Detalles Bibliográficos
Autores principales: Trejo-Cerro, Oscar, Aguilar-Hernández, Nayeli, Silva-Ayala, Daniela, López, Susana, Arias, Carlos F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173133/
https://www.ncbi.nlm.nih.gov/pubmed/30639190
http://dx.doi.org/10.1016/j.virusres.2019.01.003
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author Trejo-Cerro, Oscar
Aguilar-Hernández, Nayeli
Silva-Ayala, Daniela
López, Susana
Arias, Carlos F.
author_facet Trejo-Cerro, Oscar
Aguilar-Hernández, Nayeli
Silva-Ayala, Daniela
López, Susana
Arias, Carlos F.
author_sort Trejo-Cerro, Oscar
collection PubMed
description Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin microfilaments are important at different stages of the virus life cycle, specifically, during virus internalization and viral RNA synthesis at 6 h post-infection. Our results show that the actin-binding proteins alpha-actinin 4 and Diaph, as well as the Rho-family small GTPase Cdc42 are necessary for an efficient virus entry, while GTPase Rac1 is required for maximal viral RNA synthesis.
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spelling pubmed-71731332020-04-22 The actin cytoskeleton is important for rotavirus internalization and RNA genome replication Trejo-Cerro, Oscar Aguilar-Hernández, Nayeli Silva-Ayala, Daniela López, Susana Arias, Carlos F. Virus Res Article Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin microfilaments are important at different stages of the virus life cycle, specifically, during virus internalization and viral RNA synthesis at 6 h post-infection. Our results show that the actin-binding proteins alpha-actinin 4 and Diaph, as well as the Rho-family small GTPase Cdc42 are necessary for an efficient virus entry, while GTPase Rac1 is required for maximal viral RNA synthesis. Elsevier B.V. 2019-04-02 2019-01-09 /pmc/articles/PMC7173133/ /pubmed/30639190 http://dx.doi.org/10.1016/j.virusres.2019.01.003 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Trejo-Cerro, Oscar
Aguilar-Hernández, Nayeli
Silva-Ayala, Daniela
López, Susana
Arias, Carlos F.
The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
title The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
title_full The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
title_fullStr The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
title_full_unstemmed The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
title_short The actin cytoskeleton is important for rotavirus internalization and RNA genome replication
title_sort actin cytoskeleton is important for rotavirus internalization and rna genome replication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173133/
https://www.ncbi.nlm.nih.gov/pubmed/30639190
http://dx.doi.org/10.1016/j.virusres.2019.01.003
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