A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes

A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. The method involves virus growth in the presence of [(35)S]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guin...

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Detalles Bibliográficos
Autores principales: Cowley, J.A., Tannock, G.A., Barry, Richard D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1984
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173268/
https://www.ncbi.nlm.nih.gov/pubmed/6323512
http://dx.doi.org/10.1016/0166-0934(84)90036-3
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author Cowley, J.A.
Tannock, G.A.
Barry, Richard D.
author_facet Cowley, J.A.
Tannock, G.A.
Barry, Richard D.
author_sort Cowley, J.A.
collection PubMed
description A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. The method involves virus growth in the presence of [(35)S]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polya-crylamide gel electrophoresis (SDS-PAGE). All of the structural proteins can be detected using this procedure, and the protein profiles of virus-adsorbed erythrocyte complexes compare extremely well with those of sucrose density gradient purified virus preparations.
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spelling pubmed-71732682020-04-22 A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes Cowley, J.A. Tannock, G.A. Barry, Richard D. J Virol Methods Article A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. The method involves virus growth in the presence of [(35)S]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polya-crylamide gel electrophoresis (SDS-PAGE). All of the structural proteins can be detected using this procedure, and the protein profiles of virus-adsorbed erythrocyte complexes compare extremely well with those of sucrose density gradient purified virus preparations. Published by Elsevier B.V. 1984-02 2002-11-12 /pmc/articles/PMC7173268/ /pubmed/6323512 http://dx.doi.org/10.1016/0166-0934(84)90036-3 Text en Copyright © 1984 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Cowley, J.A.
Tannock, G.A.
Barry, Richard D.
A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
title A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
title_full A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
title_fullStr A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
title_full_unstemmed A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
title_short A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
title_sort simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173268/
https://www.ncbi.nlm.nih.gov/pubmed/6323512
http://dx.doi.org/10.1016/0166-0934(84)90036-3
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