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Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages

Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional understanding of them remains elusive. Here, by combining super-resolved localization analysis and CRISPR-C...

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Autores principales: Chong, Weng Man, Wang, Won-Jing, Lo, Chien-Hui, Chiu, Tzu-Yuan, Chang, Ting-Jui, Liu, You-Pi, Tanos, Barbara, Mazo, Gregory, Tsou, Meng-Fu Bryan, Jane, Wann-Neng, Yang, T Tony, Liao, Jung-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173962/
https://www.ncbi.nlm.nih.gov/pubmed/32242819
http://dx.doi.org/10.7554/eLife.53580
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author Chong, Weng Man
Wang, Won-Jing
Lo, Chien-Hui
Chiu, Tzu-Yuan
Chang, Ting-Jui
Liu, You-Pi
Tanos, Barbara
Mazo, Gregory
Tsou, Meng-Fu Bryan
Jane, Wann-Neng
Yang, T Tony
Liao, Jung-Chi
author_facet Chong, Weng Man
Wang, Won-Jing
Lo, Chien-Hui
Chiu, Tzu-Yuan
Chang, Ting-Jui
Liu, You-Pi
Tanos, Barbara
Mazo, Gregory
Tsou, Meng-Fu Bryan
Jane, Wann-Neng
Yang, T Tony
Liao, Jung-Chi
author_sort Chong, Weng Man
collection PubMed
description Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional understanding of them remains elusive. Here, by combining super-resolved localization analysis and CRISPR-Cas9 genetic perturbation, we find that although DAPs and sDAPs are primarily responsible for distinct functions in ciliogenesis and microtubule anchoring, respectively, the presence of one element actually affects the positioning of the other. Specifically, we find dual layers of both ODF2 and CEP89, where their localizations are differentially regulated by DAP and sDAP integrity. DAP depletion relaxes longitudinal occupancy of sDAP protein ninein to cover the DAP region, implying a role of DAPs in sDAP positioning. Removing sDAPs alter the distal border of centrosomal γ-tubulins, illustrating a new role of sDAPs. Together, our results provide an architectural framework for sDAPs that sheds light on functional understanding, surprisingly revealing coupling between DAPs and sDAPs.
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spelling pubmed-71739622020-04-23 Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages Chong, Weng Man Wang, Won-Jing Lo, Chien-Hui Chiu, Tzu-Yuan Chang, Ting-Jui Liu, You-Pi Tanos, Barbara Mazo, Gregory Tsou, Meng-Fu Bryan Jane, Wann-Neng Yang, T Tony Liao, Jung-Chi eLife Cell Biology Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional understanding of them remains elusive. Here, by combining super-resolved localization analysis and CRISPR-Cas9 genetic perturbation, we find that although DAPs and sDAPs are primarily responsible for distinct functions in ciliogenesis and microtubule anchoring, respectively, the presence of one element actually affects the positioning of the other. Specifically, we find dual layers of both ODF2 and CEP89, where their localizations are differentially regulated by DAP and sDAP integrity. DAP depletion relaxes longitudinal occupancy of sDAP protein ninein to cover the DAP region, implying a role of DAPs in sDAP positioning. Removing sDAPs alter the distal border of centrosomal γ-tubulins, illustrating a new role of sDAPs. Together, our results provide an architectural framework for sDAPs that sheds light on functional understanding, surprisingly revealing coupling between DAPs and sDAPs. eLife Sciences Publications, Ltd 2020-04-03 /pmc/articles/PMC7173962/ /pubmed/32242819 http://dx.doi.org/10.7554/eLife.53580 Text en © 2020, Chong et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Chong, Weng Man
Wang, Won-Jing
Lo, Chien-Hui
Chiu, Tzu-Yuan
Chang, Ting-Jui
Liu, You-Pi
Tanos, Barbara
Mazo, Gregory
Tsou, Meng-Fu Bryan
Jane, Wann-Neng
Yang, T Tony
Liao, Jung-Chi
Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
title Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
title_full Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
title_fullStr Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
title_full_unstemmed Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
title_short Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
title_sort super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173962/
https://www.ncbi.nlm.nih.gov/pubmed/32242819
http://dx.doi.org/10.7554/eLife.53580
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