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Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease

Few reports describe oxysterols in healthy children or in children with liver disease. We aimed to determine whether developmental changes in urinary and serum oxysterols occur during childhood, and to assess whether oxysterols might be biomarkers for pediatric liver disease. Healthy children enroll...

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Autores principales: Takaki, Yugo, Mizuochi, Tatsuki, Takei, Hajime, Eda, Keisuke, Konishi, Ken-ichiro, Ishihara, Jun, Kinoshita, Masahiro, Hashizume, Naoki, Yamashita, Yushiro, Nittono, Hiroshi, Kimura, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174363/
https://www.ncbi.nlm.nih.gov/pubmed/32317688
http://dx.doi.org/10.1038/s41598-020-63758-2
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author Takaki, Yugo
Mizuochi, Tatsuki
Takei, Hajime
Eda, Keisuke
Konishi, Ken-ichiro
Ishihara, Jun
Kinoshita, Masahiro
Hashizume, Naoki
Yamashita, Yushiro
Nittono, Hiroshi
Kimura, Akihiko
author_facet Takaki, Yugo
Mizuochi, Tatsuki
Takei, Hajime
Eda, Keisuke
Konishi, Ken-ichiro
Ishihara, Jun
Kinoshita, Masahiro
Hashizume, Naoki
Yamashita, Yushiro
Nittono, Hiroshi
Kimura, Akihiko
author_sort Takaki, Yugo
collection PubMed
description Few reports describe oxysterols in healthy children or in children with liver disease. We aimed to determine whether developmental changes in urinary and serum oxysterols occur during childhood, and to assess whether oxysterols might be biomarkers for pediatric liver disease. Healthy children enrolled as subjects (36 and 35 for urine and serum analysis, respectively) included neonates, infants, preschoolers, and school-age children, studied along with 14 healthy adults and 8 children with liver disease. We quantitated 7 oxysterols including 4β-, 20(S)-, 22(S)-, 22(R)-, 24(S)-, 25-, and 27-hydroxycholesterol using liquid chromatography/electrospray ionization-tandem mass spectrometry. Urinary total oxysterols were significantly greater in neonates than in infants (P < 0.05), preschoolers (P < 0.001), school-age children (P < 0.001), or adults (P < 0.001), declining with age. Serum total oxysterols in neonates were significantly lower than in infants (P < 0.05), preschoolers (P < 0.001), school-age children (P < 0.05), or adults (P < 0.01). Compared with healthy children, total oxysterols and 24(S)-hydroxycholesterol in liver disease were significantly increased in both urine (P < 0.001 and P < 0.001, respectively) and serum (P < 0.001 and P < 0.05, respectively). Oxysterols in liver disease, particularly 24(S)-hydroxycholesterol, were greater in urine than serum. Oxysterols change developmentally and might serve as a biomarker for pediatric liver disease. To our knowledge, this is the first such report.
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spelling pubmed-71743632020-04-24 Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease Takaki, Yugo Mizuochi, Tatsuki Takei, Hajime Eda, Keisuke Konishi, Ken-ichiro Ishihara, Jun Kinoshita, Masahiro Hashizume, Naoki Yamashita, Yushiro Nittono, Hiroshi Kimura, Akihiko Sci Rep Article Few reports describe oxysterols in healthy children or in children with liver disease. We aimed to determine whether developmental changes in urinary and serum oxysterols occur during childhood, and to assess whether oxysterols might be biomarkers for pediatric liver disease. Healthy children enrolled as subjects (36 and 35 for urine and serum analysis, respectively) included neonates, infants, preschoolers, and school-age children, studied along with 14 healthy adults and 8 children with liver disease. We quantitated 7 oxysterols including 4β-, 20(S)-, 22(S)-, 22(R)-, 24(S)-, 25-, and 27-hydroxycholesterol using liquid chromatography/electrospray ionization-tandem mass spectrometry. Urinary total oxysterols were significantly greater in neonates than in infants (P < 0.05), preschoolers (P < 0.001), school-age children (P < 0.001), or adults (P < 0.001), declining with age. Serum total oxysterols in neonates were significantly lower than in infants (P < 0.05), preschoolers (P < 0.001), school-age children (P < 0.05), or adults (P < 0.01). Compared with healthy children, total oxysterols and 24(S)-hydroxycholesterol in liver disease were significantly increased in both urine (P < 0.001 and P < 0.001, respectively) and serum (P < 0.001 and P < 0.05, respectively). Oxysterols in liver disease, particularly 24(S)-hydroxycholesterol, were greater in urine than serum. Oxysterols change developmentally and might serve as a biomarker for pediatric liver disease. To our knowledge, this is the first such report. Nature Publishing Group UK 2020-04-21 /pmc/articles/PMC7174363/ /pubmed/32317688 http://dx.doi.org/10.1038/s41598-020-63758-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Takaki, Yugo
Mizuochi, Tatsuki
Takei, Hajime
Eda, Keisuke
Konishi, Ken-ichiro
Ishihara, Jun
Kinoshita, Masahiro
Hashizume, Naoki
Yamashita, Yushiro
Nittono, Hiroshi
Kimura, Akihiko
Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
title Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
title_full Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
title_fullStr Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
title_full_unstemmed Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
title_short Urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
title_sort urinary and serum oxysterols in children: developmental pattern and potential biomarker for pediatric liver disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174363/
https://www.ncbi.nlm.nih.gov/pubmed/32317688
http://dx.doi.org/10.1038/s41598-020-63758-2
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