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“Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane
Cold seeps and hydrothermal vents deliver large amounts of methane and other gaseous alkanes into marine surface sediments. Consortia of archaea and partner bacteria thrive on the oxidation of these alkanes and its coupling to sulfate reduction. The inherently slow growth of the involved organisms a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175092/ https://www.ncbi.nlm.nih.gov/pubmed/32317322 http://dx.doi.org/10.1128/mBio.00600-20 |
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author | Hahn, Cedric Jasper Laso-Pérez, Rafael Vulcano, Francesca Vaziourakis, Konstantinos-Marios Stokke, Runar Steen, Ida Helene Teske, Andreas Boetius, Antje Liebeke, Manuel Amann, Rudolf Knittel, Katrin Wegener, Gunter |
author_facet | Hahn, Cedric Jasper Laso-Pérez, Rafael Vulcano, Francesca Vaziourakis, Konstantinos-Marios Stokke, Runar Steen, Ida Helene Teske, Andreas Boetius, Antje Liebeke, Manuel Amann, Rudolf Knittel, Katrin Wegener, Gunter |
author_sort | Hahn, Cedric Jasper |
collection | PubMed |
description | Cold seeps and hydrothermal vents deliver large amounts of methane and other gaseous alkanes into marine surface sediments. Consortia of archaea and partner bacteria thrive on the oxidation of these alkanes and its coupling to sulfate reduction. The inherently slow growth of the involved organisms and the lack of pure cultures have impeded the understanding of the molecular mechanisms of archaeal alkane degradation. Here, using hydrothermal sediments of the Guaymas Basin (Gulf of California) and ethane as the substrate, we cultured microbial consortia of a novel anaerobic ethane oxidizer, “Candidatus Ethanoperedens thermophilum” (GoM-Arc1 clade), and its partner bacterium “Candidatus Desulfofervidus auxilii,” previously known from methane-oxidizing consortia. The sulfate reduction activity of the culture doubled within one week, indicating a much faster growth than in any other alkane-oxidizing archaea described before. The dominance of a single archaeal phylotype in this culture allowed retrieval of a closed genome of “Ca. Ethanoperedens,” a sister genus of the recently reported ethane oxidizer “Candidatus Argoarchaeum.” The metagenome-assembled genome of “Ca. Ethanoperedens” encoded a complete methanogenesis pathway including a methyl-coenzyme M reductase (MCR) that is highly divergent from those of methanogens and methanotrophs. Combined substrate and metabolite analysis showed ethane as the sole growth substrate and production of ethyl-coenzyme M as the activation product. Stable isotope probing demonstrated that the enzymatic mechanism of ethane oxidation in “Ca. Ethanoperedens” is fully reversible; thus, its enzymatic machinery has potential for the biotechnological development of microbial ethane production from carbon dioxide. |
format | Online Article Text |
id | pubmed-7175092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-71750922020-04-27 “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane Hahn, Cedric Jasper Laso-Pérez, Rafael Vulcano, Francesca Vaziourakis, Konstantinos-Marios Stokke, Runar Steen, Ida Helene Teske, Andreas Boetius, Antje Liebeke, Manuel Amann, Rudolf Knittel, Katrin Wegener, Gunter mBio Research Article Cold seeps and hydrothermal vents deliver large amounts of methane and other gaseous alkanes into marine surface sediments. Consortia of archaea and partner bacteria thrive on the oxidation of these alkanes and its coupling to sulfate reduction. The inherently slow growth of the involved organisms and the lack of pure cultures have impeded the understanding of the molecular mechanisms of archaeal alkane degradation. Here, using hydrothermal sediments of the Guaymas Basin (Gulf of California) and ethane as the substrate, we cultured microbial consortia of a novel anaerobic ethane oxidizer, “Candidatus Ethanoperedens thermophilum” (GoM-Arc1 clade), and its partner bacterium “Candidatus Desulfofervidus auxilii,” previously known from methane-oxidizing consortia. The sulfate reduction activity of the culture doubled within one week, indicating a much faster growth than in any other alkane-oxidizing archaea described before. The dominance of a single archaeal phylotype in this culture allowed retrieval of a closed genome of “Ca. Ethanoperedens,” a sister genus of the recently reported ethane oxidizer “Candidatus Argoarchaeum.” The metagenome-assembled genome of “Ca. Ethanoperedens” encoded a complete methanogenesis pathway including a methyl-coenzyme M reductase (MCR) that is highly divergent from those of methanogens and methanotrophs. Combined substrate and metabolite analysis showed ethane as the sole growth substrate and production of ethyl-coenzyme M as the activation product. Stable isotope probing demonstrated that the enzymatic mechanism of ethane oxidation in “Ca. Ethanoperedens” is fully reversible; thus, its enzymatic machinery has potential for the biotechnological development of microbial ethane production from carbon dioxide. American Society for Microbiology 2020-04-21 /pmc/articles/PMC7175092/ /pubmed/32317322 http://dx.doi.org/10.1128/mBio.00600-20 Text en Copyright © 2020 Hahn et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Hahn, Cedric Jasper Laso-Pérez, Rafael Vulcano, Francesca Vaziourakis, Konstantinos-Marios Stokke, Runar Steen, Ida Helene Teske, Andreas Boetius, Antje Liebeke, Manuel Amann, Rudolf Knittel, Katrin Wegener, Gunter “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane |
title | “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane |
title_full | “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane |
title_fullStr | “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane |
title_full_unstemmed | “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane |
title_short | “Candidatus Ethanoperedens,” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane |
title_sort | “candidatus ethanoperedens,” a thermophilic genus of archaea mediating the anaerobic oxidation of ethane |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175092/ https://www.ncbi.nlm.nih.gov/pubmed/32317322 http://dx.doi.org/10.1128/mBio.00600-20 |
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