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Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine...

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Autores principales: Titsinides, Savvas, Karatzas, Theodore, Perrea, Despoina, Eleftheriadis, Efstathios, Podaropoulos, Leonidas, Kalyvas, Demos, Katopodis, Christos, Agrogiannis, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175133/
https://www.ncbi.nlm.nih.gov/pubmed/31936576
http://dx.doi.org/10.3390/dj8010007
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author Titsinides, Savvas
Karatzas, Theodore
Perrea, Despoina
Eleftheriadis, Efstathios
Podaropoulos, Leonidas
Kalyvas, Demos
Katopodis, Christos
Agrogiannis, George
author_facet Titsinides, Savvas
Karatzas, Theodore
Perrea, Despoina
Eleftheriadis, Efstathios
Podaropoulos, Leonidas
Kalyvas, Demos
Katopodis, Christos
Agrogiannis, George
author_sort Titsinides, Savvas
collection PubMed
description Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.
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spelling pubmed-71751332020-04-28 Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs Titsinides, Savvas Karatzas, Theodore Perrea, Despoina Eleftheriadis, Efstathios Podaropoulos, Leonidas Kalyvas, Demos Katopodis, Christos Agrogiannis, George Dent J (Basel) Article Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes. MDPI 2020-01-09 /pmc/articles/PMC7175133/ /pubmed/31936576 http://dx.doi.org/10.3390/dj8010007 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Titsinides, Savvas
Karatzas, Theodore
Perrea, Despoina
Eleftheriadis, Efstathios
Podaropoulos, Leonidas
Kalyvas, Demos
Katopodis, Christos
Agrogiannis, George
Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs
title Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs
title_full Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs
title_fullStr Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs
title_full_unstemmed Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs
title_short Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs
title_sort osseous healing in surgically prepared bone defects using different grafting materials: an experimental study in pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175133/
https://www.ncbi.nlm.nih.gov/pubmed/31936576
http://dx.doi.org/10.3390/dj8010007
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