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A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving t...

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Autores principales: Zouhair, Sabra, Dal Sasso, Eleonora, Tuladhar, Sugat R., Fidalgo, Catia, Vedovelli, Luca, Filippi, Andrea, Borile, Giulia, Bagno, Andrea, Marchesan, Massimo, De Rossi, Giorgio, Gregori, Dario, Wolkers, Willem F., Romanato, Filippo, Korossis, Sotirios, Gerosa, Gino, Iop, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175169/
https://www.ncbi.nlm.nih.gov/pubmed/32121155
http://dx.doi.org/10.3390/biom10030371
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author Zouhair, Sabra
Dal Sasso, Eleonora
Tuladhar, Sugat R.
Fidalgo, Catia
Vedovelli, Luca
Filippi, Andrea
Borile, Giulia
Bagno, Andrea
Marchesan, Massimo
De Rossi, Giorgio
Gregori, Dario
Wolkers, Willem F.
Romanato, Filippo
Korossis, Sotirios
Gerosa, Gino
Iop, Laura
author_facet Zouhair, Sabra
Dal Sasso, Eleonora
Tuladhar, Sugat R.
Fidalgo, Catia
Vedovelli, Luca
Filippi, Andrea
Borile, Giulia
Bagno, Andrea
Marchesan, Massimo
De Rossi, Giorgio
Gregori, Dario
Wolkers, Willem F.
Romanato, Filippo
Korossis, Sotirios
Gerosa, Gino
Iop, Laura
author_sort Zouhair, Sabra
collection PubMed
description Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.
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spelling pubmed-71751692020-04-28 A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications Zouhair, Sabra Dal Sasso, Eleonora Tuladhar, Sugat R. Fidalgo, Catia Vedovelli, Luca Filippi, Andrea Borile, Giulia Bagno, Andrea Marchesan, Massimo De Rossi, Giorgio Gregori, Dario Wolkers, Willem F. Romanato, Filippo Korossis, Sotirios Gerosa, Gino Iop, Laura Biomolecules Article Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use. MDPI 2020-02-28 /pmc/articles/PMC7175169/ /pubmed/32121155 http://dx.doi.org/10.3390/biom10030371 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zouhair, Sabra
Dal Sasso, Eleonora
Tuladhar, Sugat R.
Fidalgo, Catia
Vedovelli, Luca
Filippi, Andrea
Borile, Giulia
Bagno, Andrea
Marchesan, Massimo
De Rossi, Giorgio
Gregori, Dario
Wolkers, Willem F.
Romanato, Filippo
Korossis, Sotirios
Gerosa, Gino
Iop, Laura
A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications
title A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications
title_full A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications
title_fullStr A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications
title_full_unstemmed A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications
title_short A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications
title_sort comprehensive comparison of bovine and porcine decellularized pericardia: new insights for surgical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175169/
https://www.ncbi.nlm.nih.gov/pubmed/32121155
http://dx.doi.org/10.3390/biom10030371
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