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Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea
The cornea is the most external layer of the eye and serves two important roles in (1) the refraction of light and (2) protection from the outside environment, both of which are highly dependent on the collagen assembly of the corneal stroma. This study sought to determine the collagen fiber arrange...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175282/ https://www.ncbi.nlm.nih.gov/pubmed/31881719 http://dx.doi.org/10.3390/bioengineering7010004 |
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author | Leonard, Brian C. Cosert, Krista Winkler, Moritz Marangakis, Ariana Thomasy, Sara M. Murphy, Christopher J. Jester, James V. Raghunathan, Vijay Krishna |
author_facet | Leonard, Brian C. Cosert, Krista Winkler, Moritz Marangakis, Ariana Thomasy, Sara M. Murphy, Christopher J. Jester, James V. Raghunathan, Vijay Krishna |
author_sort | Leonard, Brian C. |
collection | PubMed |
description | The cornea is the most external layer of the eye and serves two important roles in (1) the refraction of light and (2) protection from the outside environment, both of which are highly dependent on the collagen assembly of the corneal stroma. This study sought to determine the collagen fiber arrangement of the canine corneal stroma and correlate the stromal organization with tissue stiffness in the anterior and posterior cornea. Collagen organization of the canine cornea was visualized through second-harmonic generation (SHG) imaging, and tissue stiffness of the anterior and posterior corneal stroma was determined by atomic force microscopy. Analysis of the canine anterior corneal stroma using SHG imaging documented intertwining of the collagen fibers with a high degree of fiber branching, with a more lamellar and non-branching posterior stroma. The anterior stroma had significantly higher tissue stiffness in both dogs and humans, when compared with the posterior corneal stroma (canine median: 1.3 kPa vs. 0.3 kPa; human median: 14.6 kPa vs. 2.1 kPa, respectively). There was a direct correlation between corneal collagen stromal organization and tissue stiffness in the dog, which was consistent with other mammalian species previously examined and likely reflects the need for maintenance of rigidity and corneal curvature. |
format | Online Article Text |
id | pubmed-7175282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71752822020-04-28 Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea Leonard, Brian C. Cosert, Krista Winkler, Moritz Marangakis, Ariana Thomasy, Sara M. Murphy, Christopher J. Jester, James V. Raghunathan, Vijay Krishna Bioengineering (Basel) Article The cornea is the most external layer of the eye and serves two important roles in (1) the refraction of light and (2) protection from the outside environment, both of which are highly dependent on the collagen assembly of the corneal stroma. This study sought to determine the collagen fiber arrangement of the canine corneal stroma and correlate the stromal organization with tissue stiffness in the anterior and posterior cornea. Collagen organization of the canine cornea was visualized through second-harmonic generation (SHG) imaging, and tissue stiffness of the anterior and posterior corneal stroma was determined by atomic force microscopy. Analysis of the canine anterior corneal stroma using SHG imaging documented intertwining of the collagen fibers with a high degree of fiber branching, with a more lamellar and non-branching posterior stroma. The anterior stroma had significantly higher tissue stiffness in both dogs and humans, when compared with the posterior corneal stroma (canine median: 1.3 kPa vs. 0.3 kPa; human median: 14.6 kPa vs. 2.1 kPa, respectively). There was a direct correlation between corneal collagen stromal organization and tissue stiffness in the dog, which was consistent with other mammalian species previously examined and likely reflects the need for maintenance of rigidity and corneal curvature. MDPI 2019-12-25 /pmc/articles/PMC7175282/ /pubmed/31881719 http://dx.doi.org/10.3390/bioengineering7010004 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Leonard, Brian C. Cosert, Krista Winkler, Moritz Marangakis, Ariana Thomasy, Sara M. Murphy, Christopher J. Jester, James V. Raghunathan, Vijay Krishna Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea |
title | Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea |
title_full | Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea |
title_fullStr | Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea |
title_full_unstemmed | Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea |
title_short | Stromal Collagen Arrangement Correlates with Stiffness of the Canine Cornea |
title_sort | stromal collagen arrangement correlates with stiffness of the canine cornea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175282/ https://www.ncbi.nlm.nih.gov/pubmed/31881719 http://dx.doi.org/10.3390/bioengineering7010004 |
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