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Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles
When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conf...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175343/ https://www.ncbi.nlm.nih.gov/pubmed/32155964 http://dx.doi.org/10.3390/biom10030413 |
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author | Hyltegren, Kristin Hulander, Mats Andersson, Martin Skepö, Marie |
author_facet | Hyltegren, Kristin Hulander, Mats Andersson, Martin Skepö, Marie |
author_sort | Hyltegren, Kristin |
collection | PubMed |
description | When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conformations and orientations, possibly affecting the subsequent attachment of cells to the surface. Thus, modifications of the surface nanotopography of an implant may prevent biomaterial-associated infections. Fibrinogen is of particular importance since it contains adhesion epitopes that are recognized by both eukaryotic and prokaryotic cells, and can therefore influence the adhesion of bacteria. The aim of this study was to model adsorption of fibrinogen to smooth or nanostructured silica surfaces in an attempt to further understand how surface nanotopography may affect the orientation of the adsorbed fibrinogen molecule. We used a coarse-grained model, where the main body of fibrinogen (visible in the crystal structure) was modeled as rigid and the flexible [Formula: see text] C-chains (not visible in the crystal structure) were modeled as completely disordered. We found that the elongated fibrinogen molecule preferably adsorbs in such a way that it protrudes further into solution on a nanostructured surface compared to a flat one. This implicates that the orientation on the flat surface increases its bio-availability. |
format | Online Article Text |
id | pubmed-7175343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71753432020-04-28 Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles Hyltegren, Kristin Hulander, Mats Andersson, Martin Skepö, Marie Biomolecules Article When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conformations and orientations, possibly affecting the subsequent attachment of cells to the surface. Thus, modifications of the surface nanotopography of an implant may prevent biomaterial-associated infections. Fibrinogen is of particular importance since it contains adhesion epitopes that are recognized by both eukaryotic and prokaryotic cells, and can therefore influence the adhesion of bacteria. The aim of this study was to model adsorption of fibrinogen to smooth or nanostructured silica surfaces in an attempt to further understand how surface nanotopography may affect the orientation of the adsorbed fibrinogen molecule. We used a coarse-grained model, where the main body of fibrinogen (visible in the crystal structure) was modeled as rigid and the flexible [Formula: see text] C-chains (not visible in the crystal structure) were modeled as completely disordered. We found that the elongated fibrinogen molecule preferably adsorbs in such a way that it protrudes further into solution on a nanostructured surface compared to a flat one. This implicates that the orientation on the flat surface increases its bio-availability. MDPI 2020-03-06 /pmc/articles/PMC7175343/ /pubmed/32155964 http://dx.doi.org/10.3390/biom10030413 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hyltegren, Kristin Hulander, Mats Andersson, Martin Skepö, Marie Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles |
title | Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles |
title_full | Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles |
title_fullStr | Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles |
title_full_unstemmed | Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles |
title_short | Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles |
title_sort | adsorption of fibrinogen on silica surfaces—the effect of attached nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175343/ https://www.ncbi.nlm.nih.gov/pubmed/32155964 http://dx.doi.org/10.3390/biom10030413 |
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