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RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution
In the present in vitro selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon Aeropyrum pernix. Trbp-like structures are frequently found not only in aminoacyl-tRNA synthetases but also in diverse types of proteins from different...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175363/ https://www.ncbi.nlm.nih.gov/pubmed/32024042 http://dx.doi.org/10.3390/life10020011 |
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author | Ohmori, Senri Wani, Marina Kitabatake, Saki Nakatsugawa, Yuka Ando, Tadashi Umehara, Takuya Tamura, Koji |
author_facet | Ohmori, Senri Wani, Marina Kitabatake, Saki Nakatsugawa, Yuka Ando, Tadashi Umehara, Takuya Tamura, Koji |
author_sort | Ohmori, Senri |
collection | PubMed |
description | In the present in vitro selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon Aeropyrum pernix. Trbp-like structures are frequently found not only in aminoacyl-tRNA synthetases but also in diverse types of proteins from different organisms. They likely arose early in evolution and have played important roles in evolution through interactions with key RNA structures. RNA aptamers specific for A. pernix Trbp were successfully selected from a pool of RNAs composed of 60 nucleotides, including a random 30-nucleotide region. From the secondary structures, we obtained a shortened sequence composed of 21 nucleotides, of which the 3′-terminal single stranded CA nucleotides are essential for binding. This may be related to the initial evolutionary role of the universal CCA-3′ terminus of tRNA in the interaction with Trbp-like structures. |
format | Online Article Text |
id | pubmed-7175363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71753632020-04-28 RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution Ohmori, Senri Wani, Marina Kitabatake, Saki Nakatsugawa, Yuka Ando, Tadashi Umehara, Takuya Tamura, Koji Life (Basel) Article In the present in vitro selection study, we isolated and characterized RNA aptamers for a tRNA-binding protein (Trbp) from an extremophile archaeon Aeropyrum pernix. Trbp-like structures are frequently found not only in aminoacyl-tRNA synthetases but also in diverse types of proteins from different organisms. They likely arose early in evolution and have played important roles in evolution through interactions with key RNA structures. RNA aptamers specific for A. pernix Trbp were successfully selected from a pool of RNAs composed of 60 nucleotides, including a random 30-nucleotide region. From the secondary structures, we obtained a shortened sequence composed of 21 nucleotides, of which the 3′-terminal single stranded CA nucleotides are essential for binding. This may be related to the initial evolutionary role of the universal CCA-3′ terminus of tRNA in the interaction with Trbp-like structures. MDPI 2020-02-01 /pmc/articles/PMC7175363/ /pubmed/32024042 http://dx.doi.org/10.3390/life10020011 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ohmori, Senri Wani, Marina Kitabatake, Saki Nakatsugawa, Yuka Ando, Tadashi Umehara, Takuya Tamura, Koji RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution |
title | RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution |
title_full | RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution |
title_fullStr | RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution |
title_full_unstemmed | RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution |
title_short | RNA Aptamers for a tRNA-Binding Protein from Aeropyrum pernix with Homologous Counterparts Distributed Throughout Evolution |
title_sort | rna aptamers for a trna-binding protein from aeropyrum pernix with homologous counterparts distributed throughout evolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175363/ https://www.ncbi.nlm.nih.gov/pubmed/32024042 http://dx.doi.org/10.3390/life10020011 |
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