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Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

Cordyceps militaris fruiting bodies contain a variety of bioactive components that are beneficial to the human body. However, the low yield of fruiting bodies and the low carotenoid content in C. militaris have seriously hindered the development of the C. militaris industry. To elucidate the develop...

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Autores principales: Lou, Hai-Wei, Zhao, Yu, Chen, Bai-Xiong, Yu, Ying-Hao, Tang, Hong-Biao, Ye, Zhi-Wei, Lin, Jun-Fang, Guo, Li-Qiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175373/
https://www.ncbi.nlm.nih.gov/pubmed/32155914
http://dx.doi.org/10.3390/biom10030410
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author Lou, Hai-Wei
Zhao, Yu
Chen, Bai-Xiong
Yu, Ying-Hao
Tang, Hong-Biao
Ye, Zhi-Wei
Lin, Jun-Fang
Guo, Li-Qiong
author_facet Lou, Hai-Wei
Zhao, Yu
Chen, Bai-Xiong
Yu, Ying-Hao
Tang, Hong-Biao
Ye, Zhi-Wei
Lin, Jun-Fang
Guo, Li-Qiong
author_sort Lou, Hai-Wei
collection PubMed
description Cordyceps militaris fruiting bodies contain a variety of bioactive components that are beneficial to the human body. However, the low yield of fruiting bodies and the low carotenoid content in C. militaris have seriously hindered the development of the C. militaris industry. To elucidate the developmental mechanism of the fruiting bodies of C. militaris and the biosynthesis mechanism of carotenoids, the function of the flavohemoprotein-like Cmfhp gene of C. militaris was identified for the first time. The Cmfhp gene was knocked out by the split-marker method, and the targeted gene deletion mutant ΔCmfhp was obtained. An increased nitric oxide (NO) content, no fruiting body production, decreased carotenoid content, and reduced conidial production were found in the mutant ΔCmfhp. These characteristics were restored when the Cmfhp gene expression cassette was complemented into the ΔCmfhp strain by the Agrobacterium tumefaciens-mediated transformation method. Nonetheless, the Cmfhp gene had no significant effect on the mycelial growth rate of C. militaris. These results indicated that the Cmfhp gene regulated the biosynthesis of NO and carotenoids, the development of fruiting bodies, and the formation of conidia. These findings potentially pave the way to reveal the developmental mechanism of fruiting bodies and the biosynthesis mechanism of carotenoids in C. militaris.
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spelling pubmed-71753732020-04-28 Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris Lou, Hai-Wei Zhao, Yu Chen, Bai-Xiong Yu, Ying-Hao Tang, Hong-Biao Ye, Zhi-Wei Lin, Jun-Fang Guo, Li-Qiong Biomolecules Article Cordyceps militaris fruiting bodies contain a variety of bioactive components that are beneficial to the human body. However, the low yield of fruiting bodies and the low carotenoid content in C. militaris have seriously hindered the development of the C. militaris industry. To elucidate the developmental mechanism of the fruiting bodies of C. militaris and the biosynthesis mechanism of carotenoids, the function of the flavohemoprotein-like Cmfhp gene of C. militaris was identified for the first time. The Cmfhp gene was knocked out by the split-marker method, and the targeted gene deletion mutant ΔCmfhp was obtained. An increased nitric oxide (NO) content, no fruiting body production, decreased carotenoid content, and reduced conidial production were found in the mutant ΔCmfhp. These characteristics were restored when the Cmfhp gene expression cassette was complemented into the ΔCmfhp strain by the Agrobacterium tumefaciens-mediated transformation method. Nonetheless, the Cmfhp gene had no significant effect on the mycelial growth rate of C. militaris. These results indicated that the Cmfhp gene regulated the biosynthesis of NO and carotenoids, the development of fruiting bodies, and the formation of conidia. These findings potentially pave the way to reveal the developmental mechanism of fruiting bodies and the biosynthesis mechanism of carotenoids in C. militaris. MDPI 2020-03-06 /pmc/articles/PMC7175373/ /pubmed/32155914 http://dx.doi.org/10.3390/biom10030410 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lou, Hai-Wei
Zhao, Yu
Chen, Bai-Xiong
Yu, Ying-Hao
Tang, Hong-Biao
Ye, Zhi-Wei
Lin, Jun-Fang
Guo, Li-Qiong
Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris
title Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris
title_full Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris
title_fullStr Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris
title_full_unstemmed Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris
title_short Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris
title_sort cmfhp gene mediates fruiting body development and carotenoid production in cordyceps militaris
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175373/
https://www.ncbi.nlm.nih.gov/pubmed/32155914
http://dx.doi.org/10.3390/biom10030410
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