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The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats

The mammalian target of rapamycin is not only a central regulator of lipid metabolism that controls the processes of adipogenesis and lipolysis but also a regulator of the immunometabolism of immune cells that infiltrate adipose tissue. In turn, the level of progression of diabetes is significantly...

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Autores principales: Putilin, Denis Anatolievich, Evchenko, Sergey Yuryevich, Fedoniuk, Larisa Yaroslavivna, Tokarskyy, Olexandr Stepanovich, Kamyshny, Oleksandr Mikhailovich, Migenko, Liudmyla Mikhailivna, Andreychyn, Serhiy Mikhailovich, Hanberher, Iryna Ihorivna, Bezruk, Tetyana Oleksandrivna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Carol Davila University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175427/
https://www.ncbi.nlm.nih.gov/pubmed/32341701
http://dx.doi.org/10.25122/jml-2020-0029
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author Putilin, Denis Anatolievich
Evchenko, Sergey Yuryevich
Fedoniuk, Larisa Yaroslavivna
Tokarskyy, Olexandr Stepanovich
Kamyshny, Oleksandr Mikhailovich
Migenko, Liudmyla Mikhailivna
Andreychyn, Serhiy Mikhailovich
Hanberher, Iryna Ihorivna
Bezruk, Tetyana Oleksandrivna
author_facet Putilin, Denis Anatolievich
Evchenko, Sergey Yuryevich
Fedoniuk, Larisa Yaroslavivna
Tokarskyy, Olexandr Stepanovich
Kamyshny, Oleksandr Mikhailovich
Migenko, Liudmyla Mikhailivna
Andreychyn, Serhiy Mikhailovich
Hanberher, Iryna Ihorivna
Bezruk, Tetyana Oleksandrivna
author_sort Putilin, Denis Anatolievich
collection PubMed
description The mammalian target of rapamycin is not only a central regulator of lipid metabolism that controls the processes of adipogenesis and lipolysis but also a regulator of the immunometabolism of immune cells that infiltrate adipose tissue. In turn, the level of progression of diabetes is significantly influenced by the Treg subpopulation, the complexity and heterogeneity of which is confirmed by the detection of numerous tissue-specific Tregs, including the so-called VAT Tregs (visceral adipose tissue CD4+Foxp3+ regulatory T cells). Therefore, the purpose of the study was to determine the mRNA expression levels of mTOR, Foxp3, IL1β, and IL17A genes in rat parapancreatic adipose tissue with experimental streptozotocin-induced diabetes mellitus, with or without metformin administration. The experiments were performed on male Wistar rats with induced diabetes as a result of streptozotocin administration. Molecular genetic studies were performed using real-time reverse transcription-polymerase chain reaction. The development of diabetes caused transcriptional activation of the mammalian target of rapamycin protein kinase gene, as well as increased mRNA expression of the pro-inflammatory cytokines IL1β and IL17A, but did not affect Foxp3 mRNA expression. The intervention with metformin in diabetic rats inhibited the mammalian target of rapamycin mRNA expression and caused an increase in the transcriptional activity of the Foxp3 gene in parapancreatic adipose tissue.
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spelling pubmed-71754272020-04-27 The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats Putilin, Denis Anatolievich Evchenko, Sergey Yuryevich Fedoniuk, Larisa Yaroslavivna Tokarskyy, Olexandr Stepanovich Kamyshny, Oleksandr Mikhailovich Migenko, Liudmyla Mikhailivna Andreychyn, Serhiy Mikhailovich Hanberher, Iryna Ihorivna Bezruk, Tetyana Oleksandrivna J Med Life Original Article The mammalian target of rapamycin is not only a central regulator of lipid metabolism that controls the processes of adipogenesis and lipolysis but also a regulator of the immunometabolism of immune cells that infiltrate adipose tissue. In turn, the level of progression of diabetes is significantly influenced by the Treg subpopulation, the complexity and heterogeneity of which is confirmed by the detection of numerous tissue-specific Tregs, including the so-called VAT Tregs (visceral adipose tissue CD4+Foxp3+ regulatory T cells). Therefore, the purpose of the study was to determine the mRNA expression levels of mTOR, Foxp3, IL1β, and IL17A genes in rat parapancreatic adipose tissue with experimental streptozotocin-induced diabetes mellitus, with or without metformin administration. The experiments were performed on male Wistar rats with induced diabetes as a result of streptozotocin administration. Molecular genetic studies were performed using real-time reverse transcription-polymerase chain reaction. The development of diabetes caused transcriptional activation of the mammalian target of rapamycin protein kinase gene, as well as increased mRNA expression of the pro-inflammatory cytokines IL1β and IL17A, but did not affect Foxp3 mRNA expression. The intervention with metformin in diabetic rats inhibited the mammalian target of rapamycin mRNA expression and caused an increase in the transcriptional activity of the Foxp3 gene in parapancreatic adipose tissue. Carol Davila University Press 2020 /pmc/articles/PMC7175427/ /pubmed/32341701 http://dx.doi.org/10.25122/jml-2020-0029 Text en ©Carol Davila University Press This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Original Article
Putilin, Denis Anatolievich
Evchenko, Sergey Yuryevich
Fedoniuk, Larisa Yaroslavivna
Tokarskyy, Olexandr Stepanovich
Kamyshny, Oleksandr Mikhailovich
Migenko, Liudmyla Mikhailivna
Andreychyn, Serhiy Mikhailovich
Hanberher, Iryna Ihorivna
Bezruk, Tetyana Oleksandrivna
The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats
title The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats
title_full The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats
title_fullStr The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats
title_full_unstemmed The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats
title_short The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats
title_sort influence of metformin to the transcriptional activity of the mtor and fox3 genes in parapancreatic adipose tissue of streptozotocin-induced diabetic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175427/
https://www.ncbi.nlm.nih.gov/pubmed/32341701
http://dx.doi.org/10.25122/jml-2020-0029
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