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Annotation and characterization of Babesia gibsoni apicoplast genome

BACKGROUND: Babesia gibsoni is an apicomplexan parasite transmitted by ticks, which can infect canine species and cause babesiosis. The apicoplast is an organelle associated with isoprenoids metabolism, is widely present in apicomplexan parasites, except for Cryptosporidium. Available data indicate...

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Autores principales: Liu, Qin, Yu, Long, Jiang, Fan, Li, Muxiao, Zhan, Xueyan, Huang, Yuan, Wang, Sen, Du, Xiaoyong, He, Lan, Zhao, Junlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175588/
https://www.ncbi.nlm.nih.gov/pubmed/32317011
http://dx.doi.org/10.1186/s13071-020-04065-7
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author Liu, Qin
Yu, Long
Jiang, Fan
Li, Muxiao
Zhan, Xueyan
Huang, Yuan
Wang, Sen
Du, Xiaoyong
He, Lan
Zhao, Junlong
author_facet Liu, Qin
Yu, Long
Jiang, Fan
Li, Muxiao
Zhan, Xueyan
Huang, Yuan
Wang, Sen
Du, Xiaoyong
He, Lan
Zhao, Junlong
author_sort Liu, Qin
collection PubMed
description BACKGROUND: Babesia gibsoni is an apicomplexan parasite transmitted by ticks, which can infect canine species and cause babesiosis. The apicoplast is an organelle associated with isoprenoids metabolism, is widely present in apicomplexan parasites, except for Cryptosporidium. Available data indicate that the apicoplast is essential for the survival of apicomplexan parasites. METHODS: Here, the apicoplast genome of B. gibsoni was investigated by high-throughput genome sequencing, bioinformatics analysis, and conventional PCR. RESULTS: The apicoplast genome of B. gibsoni-Wuhan strain (B. gibsoni-WH) consists of a 28.4 kb circular molecule, with A + T content of 86.33%, similar to that of B. microti. Specifically, this genome encodes genes involved in maintenance of the apicoplast DNA, transcription, translation and maturation of organellar proteins, which contains 2 subunits of ribosomal RNAs, 17 ribosomal proteins, 1 EF-Tu elongation factor (tufA), 5 DNA-dependent RNA polymerase beta subunits, 2 Clp protease chaperones, 23 tRNA genes and 5 unknown open reading frames (hypothetical proteins). Phylogenetic analysis revealed high similarity of B. gibsoni apicoplast genome to that of B. orientalis and B. bovis. CONCLUSIONS: To our knowledge, this is the first report of annotation and characterization of B. gibsoni-WH apicoplast genome. The results will facilitate the development of new anti-Babesia drug targets. [Image: see text]
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spelling pubmed-71755882020-04-24 Annotation and characterization of Babesia gibsoni apicoplast genome Liu, Qin Yu, Long Jiang, Fan Li, Muxiao Zhan, Xueyan Huang, Yuan Wang, Sen Du, Xiaoyong He, Lan Zhao, Junlong Parasit Vectors Research BACKGROUND: Babesia gibsoni is an apicomplexan parasite transmitted by ticks, which can infect canine species and cause babesiosis. The apicoplast is an organelle associated with isoprenoids metabolism, is widely present in apicomplexan parasites, except for Cryptosporidium. Available data indicate that the apicoplast is essential for the survival of apicomplexan parasites. METHODS: Here, the apicoplast genome of B. gibsoni was investigated by high-throughput genome sequencing, bioinformatics analysis, and conventional PCR. RESULTS: The apicoplast genome of B. gibsoni-Wuhan strain (B. gibsoni-WH) consists of a 28.4 kb circular molecule, with A + T content of 86.33%, similar to that of B. microti. Specifically, this genome encodes genes involved in maintenance of the apicoplast DNA, transcription, translation and maturation of organellar proteins, which contains 2 subunits of ribosomal RNAs, 17 ribosomal proteins, 1 EF-Tu elongation factor (tufA), 5 DNA-dependent RNA polymerase beta subunits, 2 Clp protease chaperones, 23 tRNA genes and 5 unknown open reading frames (hypothetical proteins). Phylogenetic analysis revealed high similarity of B. gibsoni apicoplast genome to that of B. orientalis and B. bovis. CONCLUSIONS: To our knowledge, this is the first report of annotation and characterization of B. gibsoni-WH apicoplast genome. The results will facilitate the development of new anti-Babesia drug targets. [Image: see text] BioMed Central 2020-04-21 /pmc/articles/PMC7175588/ /pubmed/32317011 http://dx.doi.org/10.1186/s13071-020-04065-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Qin
Yu, Long
Jiang, Fan
Li, Muxiao
Zhan, Xueyan
Huang, Yuan
Wang, Sen
Du, Xiaoyong
He, Lan
Zhao, Junlong
Annotation and characterization of Babesia gibsoni apicoplast genome
title Annotation and characterization of Babesia gibsoni apicoplast genome
title_full Annotation and characterization of Babesia gibsoni apicoplast genome
title_fullStr Annotation and characterization of Babesia gibsoni apicoplast genome
title_full_unstemmed Annotation and characterization of Babesia gibsoni apicoplast genome
title_short Annotation and characterization of Babesia gibsoni apicoplast genome
title_sort annotation and characterization of babesia gibsoni apicoplast genome
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175588/
https://www.ncbi.nlm.nih.gov/pubmed/32317011
http://dx.doi.org/10.1186/s13071-020-04065-7
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