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Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017
Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 dif...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176082/ https://www.ncbi.nlm.nih.gov/pubmed/32320397 http://dx.doi.org/10.1371/journal.pntd.0008165 |
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author | Zangmo, Sangay Darnal, Jit Bdr Tsheten, Gyeltshen, Sonam Thapa, Binay Thapa Rodpradit, Prinyada Chinnawirotpisan, Piyawan Manasatienkij, Wudtichai Macareo, Louis R. Fernandez, Stefan Wangchuk, Sonam Klungthong, Chonticha |
author_facet | Zangmo, Sangay Darnal, Jit Bdr Tsheten, Gyeltshen, Sonam Thapa, Binay Thapa Rodpradit, Prinyada Chinnawirotpisan, Piyawan Manasatienkij, Wudtichai Macareo, Louis R. Fernandez, Stefan Wangchuk, Sonam Klungthong, Chonticha |
author_sort | Zangmo, Sangay |
collection | PubMed |
description | Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years. |
format | Online Article Text |
id | pubmed-7176082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71760822020-04-27 Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 Zangmo, Sangay Darnal, Jit Bdr Tsheten, Gyeltshen, Sonam Thapa, Binay Thapa Rodpradit, Prinyada Chinnawirotpisan, Piyawan Manasatienkij, Wudtichai Macareo, Louis R. Fernandez, Stefan Wangchuk, Sonam Klungthong, Chonticha PLoS Negl Trop Dis Research Article Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years. Public Library of Science 2020-04-22 /pmc/articles/PMC7176082/ /pubmed/32320397 http://dx.doi.org/10.1371/journal.pntd.0008165 Text en © 2020 Zangmo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zangmo, Sangay Darnal, Jit Bdr Tsheten, Gyeltshen, Sonam Thapa, Binay Thapa Rodpradit, Prinyada Chinnawirotpisan, Piyawan Manasatienkij, Wudtichai Macareo, Louis R. Fernandez, Stefan Wangchuk, Sonam Klungthong, Chonticha Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 |
title | Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 |
title_full | Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 |
title_fullStr | Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 |
title_full_unstemmed | Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 |
title_short | Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 |
title_sort | molecular epidemiology of dengue fever outbreaks in bhutan, 2016-2017 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176082/ https://www.ncbi.nlm.nih.gov/pubmed/32320397 http://dx.doi.org/10.1371/journal.pntd.0008165 |
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