ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons

The correct subcellular distribution of proteins establishes the complex morphology and function of neurons. Fluorescence microscopy techniques are invaluable to investigate subcellular protein distribution, but they suffer from the limited ability to efficiently and reliably label endogenous protei...

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Detalles Bibliográficos
Autores principales: Willems, Jelmer, de Jong, Arthur P. H., Scheefhals, Nicky, Mertens, Eline, Catsburg, Lisa A. E., Poorthuis, Rogier B., de Winter, Fred, Verhaagen, Joost, Meye, Frank J., MacGillavry, Harold D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176289/
https://www.ncbi.nlm.nih.gov/pubmed/32275651
http://dx.doi.org/10.1371/journal.pbio.3000665

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