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Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance
Influenza A virus (FLUAV), the causative agent of influenza infection, has received extensive attention due to the recent swine-origin H1N1 pandemic. FLUAV has long been the cause of annual epidemics as well as less frequent but more severe global pandemics. Here, we describe a biosensor utilizing e...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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IEEE
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176473/ https://www.ncbi.nlm.nih.gov/pubmed/32391116 http://dx.doi.org/10.1109/TNANO.2011.2157936 |
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author | Kamikawa, Tracy L. Mikolajczyk, Malgorzata G. Kennedy, Michael Zhong, Lilin Zhang, Pei Setterington, Emma B. Scott, Dorothy E. Alocilja, Evangelyn C. |
author_facet | Kamikawa, Tracy L. Mikolajczyk, Malgorzata G. Kennedy, Michael Zhong, Lilin Zhang, Pei Setterington, Emma B. Scott, Dorothy E. Alocilja, Evangelyn C. |
author_sort | Kamikawa, Tracy L. |
collection | PubMed |
description | Influenza A virus (FLUAV), the causative agent of influenza infection, has received extensive attention due to the recent swine-origin H1N1 pandemic. FLUAV has long been the cause of annual epidemics as well as less frequent but more severe global pandemics. Here, we describe a biosensor utilizing electrically active magnetic (EAM) polyaniline-coated nanoparticles as the transducer in an electrochemical biosensor for rapidly identifying FLUAV strains based on receptor specificity, which will be useful to monitor animal influenza infections and to characterize pandemic potential of strains that have transmitted from animals to humans. Pandemic potential requires human-to-human transmissibility, which is dependent upon FLUAV hemagglutinin (HA) specificity for host glycan receptors. Avian FLUAV preferentially bind to α2,3-linked receptors, while human FLUAV bind to α2,6-linked receptors. EAM nanoparticles were prepared by synthesizing aniline monomer around gamma iron (III) oxide (γ-Fe(2)O(3)) cores, yielding 25–100-nm diameter nanoparticles that were structurally characterized by transmission electron microscopy and electron diffraction. The EAM nanoparticles were coated with monoclonal antibodies specific to H5N1 (A/Vietnam/1203/04). Specificity of binding between glycans and H5 was demonstrated. The biosensor results were correlative to supporting data from a surface plasmon resonance assay that characterized HA/glycan binding and α-H5 antibody activity. This novel study applies EAM nanoparticles as the transducer in a specific, portable, easy-to-use biosensor with great potential for disease monitoring and biosecurity applications. |
format | Online Article Text |
id | pubmed-7176473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | IEEE |
record_format | MEDLINE/PubMed |
spelling | pubmed-71764732020-05-07 Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance Kamikawa, Tracy L. Mikolajczyk, Malgorzata G. Kennedy, Michael Zhong, Lilin Zhang, Pei Setterington, Emma B. Scott, Dorothy E. Alocilja, Evangelyn C. IEEE Trans Nanotechnol Regular Papers Influenza A virus (FLUAV), the causative agent of influenza infection, has received extensive attention due to the recent swine-origin H1N1 pandemic. FLUAV has long been the cause of annual epidemics as well as less frequent but more severe global pandemics. Here, we describe a biosensor utilizing electrically active magnetic (EAM) polyaniline-coated nanoparticles as the transducer in an electrochemical biosensor for rapidly identifying FLUAV strains based on receptor specificity, which will be useful to monitor animal influenza infections and to characterize pandemic potential of strains that have transmitted from animals to humans. Pandemic potential requires human-to-human transmissibility, which is dependent upon FLUAV hemagglutinin (HA) specificity for host glycan receptors. Avian FLUAV preferentially bind to α2,3-linked receptors, while human FLUAV bind to α2,6-linked receptors. EAM nanoparticles were prepared by synthesizing aniline monomer around gamma iron (III) oxide (γ-Fe(2)O(3)) cores, yielding 25–100-nm diameter nanoparticles that were structurally characterized by transmission electron microscopy and electron diffraction. The EAM nanoparticles were coated with monoclonal antibodies specific to H5N1 (A/Vietnam/1203/04). Specificity of binding between glycans and H5 was demonstrated. The biosensor results were correlative to supporting data from a surface plasmon resonance assay that characterized HA/glycan binding and α-H5 antibody activity. This novel study applies EAM nanoparticles as the transducer in a specific, portable, easy-to-use biosensor with great potential for disease monitoring and biosecurity applications. IEEE 2011-05-27 /pmc/articles/PMC7176473/ /pubmed/32391116 http://dx.doi.org/10.1109/TNANO.2011.2157936 Text en https://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 License. For more information, see https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Regular Papers Kamikawa, Tracy L. Mikolajczyk, Malgorzata G. Kennedy, Michael Zhong, Lilin Zhang, Pei Setterington, Emma B. Scott, Dorothy E. Alocilja, Evangelyn C. Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance |
title | Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance |
title_full | Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance |
title_fullStr | Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance |
title_full_unstemmed | Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance |
title_short | Pandemic Influenza Detection by Electrically Active Magnetic Nanoparticles and Surface Plasmon Resonance |
title_sort | pandemic influenza detection by electrically active magnetic nanoparticles and surface plasmon resonance |
topic | Regular Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176473/ https://www.ncbi.nlm.nih.gov/pubmed/32391116 http://dx.doi.org/10.1109/TNANO.2011.2157936 |
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