A modified method for purification of Eimeria tenella sporozoites

Coccidiosis is an economically important gastrointestinal disease in domestic fowl. Eimeria species are the causative agents of avian coccidiosis. Current challenges in management and prevention of eimeriosis enhance the need for research in this field. Sporozoite purification is a necessary step fo...

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Autores principales: Rentería-Solís, Zaida, Zhang, Runhui, Taha, Shahinaz, Daugschies, Arwid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Protozoology - Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176601/
https://www.ncbi.nlm.nih.gov/pubmed/31942639
http://dx.doi.org/10.1007/s00436-020-06602-w
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author Rentería-Solís, Zaida
Zhang, Runhui
Taha, Shahinaz
Daugschies, Arwid
author_facet Rentería-Solís, Zaida
Zhang, Runhui
Taha, Shahinaz
Daugschies, Arwid
author_sort Rentería-Solís, Zaida
collection PubMed
description Coccidiosis is an economically important gastrointestinal disease in domestic fowl. Eimeria species are the causative agents of avian coccidiosis. Current challenges in management and prevention of eimeriosis enhance the need for research in this field. Sporozoite purification is a necessary step for Eimeria spp. in vitro infection models. Current alternatives such as DE-52 anion exchange chromatography and Percoll gradient require time and resources. We present a modified protocol consisting on vacuum filtration of sporozoites using a disposable 5-μL filter. Yield percentages were similar to those reported for Percoll gradient purification. By reducing time and efforts during sporozoite purification, it could be possible to increase resources in other areas of Eimeria studies.
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spelling pubmed-71766012020-04-28 A modified method for purification of Eimeria tenella sporozoites Rentería-Solís, Zaida Zhang, Runhui Taha, Shahinaz Daugschies, Arwid Parasitol Res Protozoology - Short Communication Coccidiosis is an economically important gastrointestinal disease in domestic fowl. Eimeria species are the causative agents of avian coccidiosis. Current challenges in management and prevention of eimeriosis enhance the need for research in this field. Sporozoite purification is a necessary step for Eimeria spp. in vitro infection models. Current alternatives such as DE-52 anion exchange chromatography and Percoll gradient require time and resources. We present a modified protocol consisting on vacuum filtration of sporozoites using a disposable 5-μL filter. Yield percentages were similar to those reported for Percoll gradient purification. By reducing time and efforts during sporozoite purification, it could be possible to increase resources in other areas of Eimeria studies. Springer Berlin Heidelberg 2020-01-16 2020 /pmc/articles/PMC7176601/ /pubmed/31942639 http://dx.doi.org/10.1007/s00436-020-06602-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Protozoology - Short Communication
Rentería-Solís, Zaida
Zhang, Runhui
Taha, Shahinaz
Daugschies, Arwid
A modified method for purification of Eimeria tenella sporozoites
title A modified method for purification of Eimeria tenella sporozoites
title_full A modified method for purification of Eimeria tenella sporozoites
title_fullStr A modified method for purification of Eimeria tenella sporozoites
title_full_unstemmed A modified method for purification of Eimeria tenella sporozoites
title_short A modified method for purification of Eimeria tenella sporozoites
title_sort modified method for purification of eimeria tenella sporozoites
topic Protozoology - Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176601/
https://www.ncbi.nlm.nih.gov/pubmed/31942639
http://dx.doi.org/10.1007/s00436-020-06602-w
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