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CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases

Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and thei...

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Autores principales: Li, Xue, Xu, Ao, Li, Kai, Zhang, Jie, Li, Qin, Zhao, Gang, Zhang, Yue, Yuan, Hang, Guo, Yafei, Lin, Ping, Huang, Lugang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176872/
https://www.ncbi.nlm.nih.gov/pubmed/32181567
http://dx.doi.org/10.1111/jcmm.15128
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author Li, Xue
Xu, Ao
Li, Kai
Zhang, Jie
Li, Qin
Zhao, Gang
Zhang, Yue
Yuan, Hang
Guo, Yafei
Lin, Ping
Huang, Lugang
author_facet Li, Xue
Xu, Ao
Li, Kai
Zhang, Jie
Li, Qin
Zhao, Gang
Zhang, Yue
Yuan, Hang
Guo, Yafei
Lin, Ping
Huang, Lugang
author_sort Li, Xue
collection PubMed
description Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29(+) CD44(+) CD34(−) CD45(−) ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases.
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spelling pubmed-71768722020-04-24 CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases Li, Xue Xu, Ao Li, Kai Zhang, Jie Li, Qin Zhao, Gang Zhang, Yue Yuan, Hang Guo, Yafei Lin, Ping Huang, Lugang J Cell Mol Med Original Articles Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29(+) CD44(+) CD34(−) CD45(−) ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases. John Wiley and Sons Inc. 2020-03-17 2020-04 /pmc/articles/PMC7176872/ /pubmed/32181567 http://dx.doi.org/10.1111/jcmm.15128 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Li, Xue
Xu, Ao
Li, Kai
Zhang, Jie
Li, Qin
Zhao, Gang
Zhang, Yue
Yuan, Hang
Guo, Yafei
Lin, Ping
Huang, Lugang
CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
title CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
title_full CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
title_fullStr CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
title_full_unstemmed CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
title_short CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
title_sort cxcr4‐sf1 bifunctional adipose‐derived stem cells benefit for the treatment of leydig cell dysfunction‐related diseases
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176872/
https://www.ncbi.nlm.nih.gov/pubmed/32181567
http://dx.doi.org/10.1111/jcmm.15128
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