Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors

A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the...

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Detalles Bibliográficos
Autores principales: Khazak, Vladimir, Golemis, Erica A., Weber, Lutz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177006/
https://www.ncbi.nlm.nih.gov/pubmed/16353379
http://dx.doi.org/10.1007/978-1-59259-948-6_18
Descripción
Sumario:A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.

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