Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors

A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the...

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Autores principales: Khazak, Vladimir, Golemis, Erica A., Weber, Lutz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177006/
https://www.ncbi.nlm.nih.gov/pubmed/16353379
http://dx.doi.org/10.1007/978-1-59259-948-6_18
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author Khazak, Vladimir
Golemis, Erica A.
Weber, Lutz
author_facet Khazak, Vladimir
Golemis, Erica A.
Weber, Lutz
author_sort Khazak, Vladimir
collection PubMed
description A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
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spelling pubmed-71770062020-04-23 Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors Khazak, Vladimir Golemis, Erica A. Weber, Lutz Chemical Genomics Article A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors. 2005 /pmc/articles/PMC7177006/ /pubmed/16353379 http://dx.doi.org/10.1007/978-1-59259-948-6_18 Text en © Humana Press Inc. 2005 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Khazak, Vladimir
Golemis, Erica A.
Weber, Lutz
Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
title Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
title_full Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
title_fullStr Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
title_full_unstemmed Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
title_short Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors
title_sort development of a yeast two-hybrid screen for selection of human ras-raf protein interaction inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177006/
https://www.ncbi.nlm.nih.gov/pubmed/16353379
http://dx.doi.org/10.1007/978-1-59259-948-6_18
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