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Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi
CRISPR/Cas9 technology is a powerful tool for the design of gene-drive systems to control and/or modify mosquito vector populations; however, CRISPR/Cas9-mediated nonhomologous end joining mutations can have an important impact on generating alleles resistant to the drive and thus on drive efficienc...
Autores principales: | Carballar-Lejarazú, Rebeca, Kelsey, Adam, Pham, Thai Binh, Bennett, Eric P, James, Anthony A |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177198/ https://www.ncbi.nlm.nih.gov/pubmed/32040336 http://dx.doi.org/10.2144/btn-2019-0103 |
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