A cell-based assay for the detection of neutralizing antibodies against alemtuzumab

AIM: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. RESULTS: Alemtuzumab–Alexa Fl...

Descripción completa

Detalles Bibliográficos
Autores principales: Ali, Liaqat, Saxena, Gauri, Jones, Meleri, Leisegang, Georgia R, Gammon, Luke, Gnanapavan, Sharmilee, Giovannoni, Gavin, Schmierer, Klaus, Baker, David, Kang, Angray S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177201/
https://www.ncbi.nlm.nih.gov/pubmed/32096651
http://dx.doi.org/10.2144/btn-2019-0122
_version_ 1783525166628732928
author Ali, Liaqat
Saxena, Gauri
Jones, Meleri
Leisegang, Georgia R
Gammon, Luke
Gnanapavan, Sharmilee
Giovannoni, Gavin
Schmierer, Klaus
Baker, David
Kang, Angray S
author_facet Ali, Liaqat
Saxena, Gauri
Jones, Meleri
Leisegang, Georgia R
Gammon, Luke
Gnanapavan, Sharmilee
Giovannoni, Gavin
Schmierer, Klaus
Baker, David
Kang, Angray S
author_sort Ali, Liaqat
collection PubMed
description AIM: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. RESULTS: Alemtuzumab–Alexa Fluor 488 conjugate binding to the CHO-CD52 cell surface was inhibited by anti-alemtuzumab antibodies. CONCLUSION: In this proof-of-concept study, a CHO-CD52 cell line has been developed and used to detect the presence of anti-alemtuzumab neutralizing antibodies. This platform provides the basis of an assay for routine screening of serum for neutralizing antibodies from patients treated with alemtuzumab.
format Online
Article
Text
id pubmed-7177201
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Future Science Ltd
record_format MEDLINE/PubMed
spelling pubmed-71772012020-04-28 A cell-based assay for the detection of neutralizing antibodies against alemtuzumab Ali, Liaqat Saxena, Gauri Jones, Meleri Leisegang, Georgia R Gammon, Luke Gnanapavan, Sharmilee Giovannoni, Gavin Schmierer, Klaus Baker, David Kang, Angray S Biotechniques Reports AIM: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. RESULTS: Alemtuzumab–Alexa Fluor 488 conjugate binding to the CHO-CD52 cell surface was inhibited by anti-alemtuzumab antibodies. CONCLUSION: In this proof-of-concept study, a CHO-CD52 cell line has been developed and used to detect the presence of anti-alemtuzumab neutralizing antibodies. This platform provides the basis of an assay for routine screening of serum for neutralizing antibodies from patients treated with alemtuzumab. Future Science Ltd 2020-02-25 2020-04 /pmc/articles/PMC7177201/ /pubmed/32096651 http://dx.doi.org/10.2144/btn-2019-0122 Text en © 2020 Angray S Kang This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Reports
Ali, Liaqat
Saxena, Gauri
Jones, Meleri
Leisegang, Georgia R
Gammon, Luke
Gnanapavan, Sharmilee
Giovannoni, Gavin
Schmierer, Klaus
Baker, David
Kang, Angray S
A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
title A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
title_full A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
title_fullStr A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
title_full_unstemmed A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
title_short A cell-based assay for the detection of neutralizing antibodies against alemtuzumab
title_sort cell-based assay for the detection of neutralizing antibodies against alemtuzumab
topic Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177201/
https://www.ncbi.nlm.nih.gov/pubmed/32096651
http://dx.doi.org/10.2144/btn-2019-0122
work_keys_str_mv AT aliliaqat acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT saxenagauri acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT jonesmeleri acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT leiseganggeorgiar acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT gammonluke acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT gnanapavansharmilee acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT giovannonigavin acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT schmiererklaus acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT bakerdavid acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT kangangrays acellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT aliliaqat cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT saxenagauri cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT jonesmeleri cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT leiseganggeorgiar cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT gammonluke cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT gnanapavansharmilee cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT giovannonigavin cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT schmiererklaus cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT bakerdavid cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab
AT kangangrays cellbasedassayforthedetectionofneutralizingantibodiesagainstalemtuzumab

Ejemplares similares