Cargando…
Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177269/ https://www.ncbi.nlm.nih.gov/pubmed/32260390 http://dx.doi.org/10.3390/ijms21072510 |
_version_ | 1783525181103276032 |
---|---|
author | Piwowar, Agnieszka Rembiałkowska, Nina Rorbach-Dolata, Anna Garbiec, Arnold Ślusarczyk, Sylwester Dobosz, Agnieszka Długosz, Anna Marchewka, Zofia Matkowski, Adam Saczko, Jolanta |
author_facet | Piwowar, Agnieszka Rembiałkowska, Nina Rorbach-Dolata, Anna Garbiec, Arnold Ślusarczyk, Sylwester Dobosz, Agnieszka Długosz, Anna Marchewka, Zofia Matkowski, Adam Saczko, Jolanta |
author_sort | Piwowar, Agnieszka |
collection | PubMed |
description | The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line exposed to the neurotoxic agent—3-nitropropionic acid (3-NP). The xanthone-enriched fraction of the ethanolic extract of A. asphodeloides (abbreviated from now on as XF, for the Xanthone Fraction), rich in polyphenolic xanthone glycosides, in concentrations from 5 to 100 μg/mL, and 3-NP in concentrations from 2.5 to 15 mM, were examined. After 8, 16, 24, 48, and 72 h of exposure of cells to various combinations of 3-NP and XF, the MTT viability assay was performed and morphological changes were estimated by confocal fluorescence microscopy. The obtained results showed a significant increase in the number of cells surviving after treatment with XF with exposure to neurotoxic 3-NP and decreased morphological changes in PC12 cells in a dose and time dependent manner. The most effective protective action was observed when PC12 cells were pre-incubated with the XF. This effect may contribute to the traditional indications of this herb for neurological and cognitive complaints. However, a significant cytotoxicity observed at higher XF concentrations (over 10 µg/mL) and longer incubation time (48 h) requires caution in future research and thorough investigation into potential adverse effects. |
format | Online Article Text |
id | pubmed-7177269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71772692020-04-28 Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid Piwowar, Agnieszka Rembiałkowska, Nina Rorbach-Dolata, Anna Garbiec, Arnold Ślusarczyk, Sylwester Dobosz, Agnieszka Długosz, Anna Marchewka, Zofia Matkowski, Adam Saczko, Jolanta Int J Mol Sci Article The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line exposed to the neurotoxic agent—3-nitropropionic acid (3-NP). The xanthone-enriched fraction of the ethanolic extract of A. asphodeloides (abbreviated from now on as XF, for the Xanthone Fraction), rich in polyphenolic xanthone glycosides, in concentrations from 5 to 100 μg/mL, and 3-NP in concentrations from 2.5 to 15 mM, were examined. After 8, 16, 24, 48, and 72 h of exposure of cells to various combinations of 3-NP and XF, the MTT viability assay was performed and morphological changes were estimated by confocal fluorescence microscopy. The obtained results showed a significant increase in the number of cells surviving after treatment with XF with exposure to neurotoxic 3-NP and decreased morphological changes in PC12 cells in a dose and time dependent manner. The most effective protective action was observed when PC12 cells were pre-incubated with the XF. This effect may contribute to the traditional indications of this herb for neurological and cognitive complaints. However, a significant cytotoxicity observed at higher XF concentrations (over 10 µg/mL) and longer incubation time (48 h) requires caution in future research and thorough investigation into potential adverse effects. MDPI 2020-04-04 /pmc/articles/PMC7177269/ /pubmed/32260390 http://dx.doi.org/10.3390/ijms21072510 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Piwowar, Agnieszka Rembiałkowska, Nina Rorbach-Dolata, Anna Garbiec, Arnold Ślusarczyk, Sylwester Dobosz, Agnieszka Długosz, Anna Marchewka, Zofia Matkowski, Adam Saczko, Jolanta Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid |
title | Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid |
title_full | Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid |
title_fullStr | Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid |
title_full_unstemmed | Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid |
title_short | Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid |
title_sort | anemarrhenae asphodeloides rhizoma extract enriched in mangiferin protects pc12 cells against a neurotoxic agent-3-nitropropionic acid |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177269/ https://www.ncbi.nlm.nih.gov/pubmed/32260390 http://dx.doi.org/10.3390/ijms21072510 |
work_keys_str_mv | AT piwowaragnieszka anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT rembiałkowskanina anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT rorbachdolataanna anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT garbiecarnold anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT slusarczyksylwester anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT doboszagnieszka anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT długoszanna anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT marchewkazofia anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT matkowskiadam anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid AT saczkojolanta anemarrhenaeasphodeloidesrhizomaextractenrichedinmangiferinprotectspc12cellsagainstaneurotoxicagent3nitropropionicacid |