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Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid

The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line...

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Autores principales: Piwowar, Agnieszka, Rembiałkowska, Nina, Rorbach-Dolata, Anna, Garbiec, Arnold, Ślusarczyk, Sylwester, Dobosz, Agnieszka, Długosz, Anna, Marchewka, Zofia, Matkowski, Adam, Saczko, Jolanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177269/
https://www.ncbi.nlm.nih.gov/pubmed/32260390
http://dx.doi.org/10.3390/ijms21072510
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author Piwowar, Agnieszka
Rembiałkowska, Nina
Rorbach-Dolata, Anna
Garbiec, Arnold
Ślusarczyk, Sylwester
Dobosz, Agnieszka
Długosz, Anna
Marchewka, Zofia
Matkowski, Adam
Saczko, Jolanta
author_facet Piwowar, Agnieszka
Rembiałkowska, Nina
Rorbach-Dolata, Anna
Garbiec, Arnold
Ślusarczyk, Sylwester
Dobosz, Agnieszka
Długosz, Anna
Marchewka, Zofia
Matkowski, Adam
Saczko, Jolanta
author_sort Piwowar, Agnieszka
collection PubMed
description The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line exposed to the neurotoxic agent—3-nitropropionic acid (3-NP). The xanthone-enriched fraction of the ethanolic extract of A. asphodeloides (abbreviated from now on as XF, for the Xanthone Fraction), rich in polyphenolic xanthone glycosides, in concentrations from 5 to 100 μg/mL, and 3-NP in concentrations from 2.5 to 15 mM, were examined. After 8, 16, 24, 48, and 72 h of exposure of cells to various combinations of 3-NP and XF, the MTT viability assay was performed and morphological changes were estimated by confocal fluorescence microscopy. The obtained results showed a significant increase in the number of cells surviving after treatment with XF with exposure to neurotoxic 3-NP and decreased morphological changes in PC12 cells in a dose and time dependent manner. The most effective protective action was observed when PC12 cells were pre-incubated with the XF. This effect may contribute to the traditional indications of this herb for neurological and cognitive complaints. However, a significant cytotoxicity observed at higher XF concentrations (over 10 µg/mL) and longer incubation time (48 h) requires caution in future research and thorough investigation into potential adverse effects.
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spelling pubmed-71772692020-04-28 Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid Piwowar, Agnieszka Rembiałkowska, Nina Rorbach-Dolata, Anna Garbiec, Arnold Ślusarczyk, Sylwester Dobosz, Agnieszka Długosz, Anna Marchewka, Zofia Matkowski, Adam Saczko, Jolanta Int J Mol Sci Article The rhizome of Anemarrhena asphodeloides Bunge, used in Traditional Chinese Medicine as a brain function-improving herb, is a promising source of neuroprotective substances. The aim of this study was to evaluate the protective action of xanthones from A. asphodeloides rhizomes on the PC12 cell line exposed to the neurotoxic agent—3-nitropropionic acid (3-NP). The xanthone-enriched fraction of the ethanolic extract of A. asphodeloides (abbreviated from now on as XF, for the Xanthone Fraction), rich in polyphenolic xanthone glycosides, in concentrations from 5 to 100 μg/mL, and 3-NP in concentrations from 2.5 to 15 mM, were examined. After 8, 16, 24, 48, and 72 h of exposure of cells to various combinations of 3-NP and XF, the MTT viability assay was performed and morphological changes were estimated by confocal fluorescence microscopy. The obtained results showed a significant increase in the number of cells surviving after treatment with XF with exposure to neurotoxic 3-NP and decreased morphological changes in PC12 cells in a dose and time dependent manner. The most effective protective action was observed when PC12 cells were pre-incubated with the XF. This effect may contribute to the traditional indications of this herb for neurological and cognitive complaints. However, a significant cytotoxicity observed at higher XF concentrations (over 10 µg/mL) and longer incubation time (48 h) requires caution in future research and thorough investigation into potential adverse effects. MDPI 2020-04-04 /pmc/articles/PMC7177269/ /pubmed/32260390 http://dx.doi.org/10.3390/ijms21072510 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Piwowar, Agnieszka
Rembiałkowska, Nina
Rorbach-Dolata, Anna
Garbiec, Arnold
Ślusarczyk, Sylwester
Dobosz, Agnieszka
Długosz, Anna
Marchewka, Zofia
Matkowski, Adam
Saczko, Jolanta
Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
title Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
title_full Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
title_fullStr Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
title_full_unstemmed Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
title_short Anemarrhenae asphodeloides rhizoma Extract Enriched in Mangiferin Protects PC12 Cells against a Neurotoxic Agent-3-Nitropropionic Acid
title_sort anemarrhenae asphodeloides rhizoma extract enriched in mangiferin protects pc12 cells against a neurotoxic agent-3-nitropropionic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177269/
https://www.ncbi.nlm.nih.gov/pubmed/32260390
http://dx.doi.org/10.3390/ijms21072510
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