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Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis

Endometriosis is a gynecological condition that is associated with chronic pelvic inflammation, pain, and infertility. Although substantial evidence supports that immunological alterations contribute to its pathogenesis and we previously posed a pivotal role of Galectin-9 (Gal-9) in this disorder, t...

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Autores principales: Meggyes, Matyas, Szereday, Laszlo, Bohonyi, Noemi, Koppan, Miklos, Szegedi, Sarolta, Marics-Kutas, Anna, Marton, Mirjam, Totsimon, Anett, Polgar, Beata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177301/
https://www.ncbi.nlm.nih.gov/pubmed/32231038
http://dx.doi.org/10.3390/ijms21072343
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author Meggyes, Matyas
Szereday, Laszlo
Bohonyi, Noemi
Koppan, Miklos
Szegedi, Sarolta
Marics-Kutas, Anna
Marton, Mirjam
Totsimon, Anett
Polgar, Beata
author_facet Meggyes, Matyas
Szereday, Laszlo
Bohonyi, Noemi
Koppan, Miklos
Szegedi, Sarolta
Marics-Kutas, Anna
Marton, Mirjam
Totsimon, Anett
Polgar, Beata
author_sort Meggyes, Matyas
collection PubMed
description Endometriosis is a gynecological condition that is associated with chronic pelvic inflammation, pain, and infertility. Although substantial evidence supports that immunological alterations contribute to its pathogenesis and we previously posed a pivotal role of Galectin-9 (Gal-9) in this disorder, the involvement of the TIM-3/Gal-9 pathway in the development of endometriosis-associated immunological abnormalities is not yet known. In the present study, multicolor flow cytometry was used to compare the immunophenotype and cell surface expression of TIM-3 and Gal-9 molecules on peripheral blood (PB) and peritoneal fluid (PF) lymphocytes of women with and without endometriosis. We found an altered distribution of different lymphocyte subpopulations, a markedly decreased TIM-3 labeling on all T and NK subsets and a significantly increased Gal-9 positivity on peripheral CD4+ T and Treg cells of the affected cohort. Furthermore, a significantly increased TIM-3 expression on CD4+T-cells and elevated Gal-9 labeling on all T and NK subsets was also revealed in the PF of the examined patients. In conclusion, our results suggest a persistent activation and disturbed TIM-3/Gal-9-dependent regulatory function in endometriosis, which may be involved in the impaired immune surveillance mechanisms, promotes the survival of ectopic lesions, and aids the evolution of reproductive failures in endometriosis.
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spelling pubmed-71773012020-04-28 Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis Meggyes, Matyas Szereday, Laszlo Bohonyi, Noemi Koppan, Miklos Szegedi, Sarolta Marics-Kutas, Anna Marton, Mirjam Totsimon, Anett Polgar, Beata Int J Mol Sci Article Endometriosis is a gynecological condition that is associated with chronic pelvic inflammation, pain, and infertility. Although substantial evidence supports that immunological alterations contribute to its pathogenesis and we previously posed a pivotal role of Galectin-9 (Gal-9) in this disorder, the involvement of the TIM-3/Gal-9 pathway in the development of endometriosis-associated immunological abnormalities is not yet known. In the present study, multicolor flow cytometry was used to compare the immunophenotype and cell surface expression of TIM-3 and Gal-9 molecules on peripheral blood (PB) and peritoneal fluid (PF) lymphocytes of women with and without endometriosis. We found an altered distribution of different lymphocyte subpopulations, a markedly decreased TIM-3 labeling on all T and NK subsets and a significantly increased Gal-9 positivity on peripheral CD4+ T and Treg cells of the affected cohort. Furthermore, a significantly increased TIM-3 expression on CD4+T-cells and elevated Gal-9 labeling on all T and NK subsets was also revealed in the PF of the examined patients. In conclusion, our results suggest a persistent activation and disturbed TIM-3/Gal-9-dependent regulatory function in endometriosis, which may be involved in the impaired immune surveillance mechanisms, promotes the survival of ectopic lesions, and aids the evolution of reproductive failures in endometriosis. MDPI 2020-03-28 /pmc/articles/PMC7177301/ /pubmed/32231038 http://dx.doi.org/10.3390/ijms21072343 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meggyes, Matyas
Szereday, Laszlo
Bohonyi, Noemi
Koppan, Miklos
Szegedi, Sarolta
Marics-Kutas, Anna
Marton, Mirjam
Totsimon, Anett
Polgar, Beata
Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
title Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
title_full Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
title_fullStr Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
title_full_unstemmed Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
title_short Different Expression Pattern of TIM-3 and Galectin-9 Molecules by Peripheral and Peritoneal Lymphocytes in Women with and without Endometriosis
title_sort different expression pattern of tim-3 and galectin-9 molecules by peripheral and peritoneal lymphocytes in women with and without endometriosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177301/
https://www.ncbi.nlm.nih.gov/pubmed/32231038
http://dx.doi.org/10.3390/ijms21072343
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