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Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis

Gestational diabetes mellitus (GDM) is the most common pregnancy-associated metabolic disorder that negatively impacts on the health of both mothers and their offspring in the long-term. The molecular mechanisms involved are not fully understood. As in other states of insulin resistance, a dispropor...

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Autores principales: Hepp, Paula, Unverdorben, Laura, Hutter, Stefan, Kuhn, Christina, Ditsch, Nina, Groß, Eva, Mahner, Sven, Jeschke, Udo, Knabl, Julia, Heidegger, Helene H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177347/
https://www.ncbi.nlm.nih.gov/pubmed/32244351
http://dx.doi.org/10.3390/ijms21072404
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author Hepp, Paula
Unverdorben, Laura
Hutter, Stefan
Kuhn, Christina
Ditsch, Nina
Groß, Eva
Mahner, Sven
Jeschke, Udo
Knabl, Julia
Heidegger, Helene H.
author_facet Hepp, Paula
Unverdorben, Laura
Hutter, Stefan
Kuhn, Christina
Ditsch, Nina
Groß, Eva
Mahner, Sven
Jeschke, Udo
Knabl, Julia
Heidegger, Helene H.
author_sort Hepp, Paula
collection PubMed
description Gestational diabetes mellitus (GDM) is the most common pregnancy-associated metabolic disorder that negatively impacts on the health of both mothers and their offspring in the long-term. The molecular mechanisms involved are not fully understood. As in other states of insulin resistance, a disproportionate immune response in GDM leads to a state of chronic low-grade inflammation. Galectin-2 exerts regulatory effects on different immune cells. This study investigated galectin-2 expression in the placenta of 40 GDM patients and 40 controls, in a sex-specific manner. Immunohistochemistry was used for semi-quantitative analysis of expression strength. The phenotypes of galectin-2 expressing cells were characterized through double immunofluorescence. We found a significant up-regulation of galectin-2 in the fetal syncytiotrophoblast, as well as in the maternal decidua of GDM placentas. Double staining showed a strong galectin-2 expression in extra villous trophoblast cells and fetal endothelial cells in GDM. These findings present the first systematic investigation of galectin-2 in GDM. The findings contribute to the emerging understanding of the role of immunomodulation and inflammation in GDM and of galectin-2 itself. This might also have implications for the long-term cardiovascular health of the offspring.
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spelling pubmed-71773472020-04-28 Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis Hepp, Paula Unverdorben, Laura Hutter, Stefan Kuhn, Christina Ditsch, Nina Groß, Eva Mahner, Sven Jeschke, Udo Knabl, Julia Heidegger, Helene H. Int J Mol Sci Article Gestational diabetes mellitus (GDM) is the most common pregnancy-associated metabolic disorder that negatively impacts on the health of both mothers and their offspring in the long-term. The molecular mechanisms involved are not fully understood. As in other states of insulin resistance, a disproportionate immune response in GDM leads to a state of chronic low-grade inflammation. Galectin-2 exerts regulatory effects on different immune cells. This study investigated galectin-2 expression in the placenta of 40 GDM patients and 40 controls, in a sex-specific manner. Immunohistochemistry was used for semi-quantitative analysis of expression strength. The phenotypes of galectin-2 expressing cells were characterized through double immunofluorescence. We found a significant up-regulation of galectin-2 in the fetal syncytiotrophoblast, as well as in the maternal decidua of GDM placentas. Double staining showed a strong galectin-2 expression in extra villous trophoblast cells and fetal endothelial cells in GDM. These findings present the first systematic investigation of galectin-2 in GDM. The findings contribute to the emerging understanding of the role of immunomodulation and inflammation in GDM and of galectin-2 itself. This might also have implications for the long-term cardiovascular health of the offspring. MDPI 2020-03-31 /pmc/articles/PMC7177347/ /pubmed/32244351 http://dx.doi.org/10.3390/ijms21072404 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hepp, Paula
Unverdorben, Laura
Hutter, Stefan
Kuhn, Christina
Ditsch, Nina
Groß, Eva
Mahner, Sven
Jeschke, Udo
Knabl, Julia
Heidegger, Helene H.
Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis
title Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis
title_full Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis
title_fullStr Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis
title_full_unstemmed Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis
title_short Placental Galectin-2 Expression in Gestational Diabetes: A Systematic, Histological Analysis
title_sort placental galectin-2 expression in gestational diabetes: a systematic, histological analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177347/
https://www.ncbi.nlm.nih.gov/pubmed/32244351
http://dx.doi.org/10.3390/ijms21072404
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