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Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings

Bacterial infection associated with medical implants is a major threat to healthcare. This work reports the fabrication of Copper(II)–Chitosan (Cu(II)–CS) complex coatings deposited by electrophoretic deposition (EPD) as potential antibacterial candidate to combat microorganisms to reduce implant re...

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Autores principales: Akhtar, Muhammad Asim, Ilyas, Kanwal, Dlouhý, Ivo, Siska, Filip, Boccaccini, Aldo R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177350/
https://www.ncbi.nlm.nih.gov/pubmed/32290155
http://dx.doi.org/10.3390/ijms21072637
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author Akhtar, Muhammad Asim
Ilyas, Kanwal
Dlouhý, Ivo
Siska, Filip
Boccaccini, Aldo R.
author_facet Akhtar, Muhammad Asim
Ilyas, Kanwal
Dlouhý, Ivo
Siska, Filip
Boccaccini, Aldo R.
author_sort Akhtar, Muhammad Asim
collection PubMed
description Bacterial infection associated with medical implants is a major threat to healthcare. This work reports the fabrication of Copper(II)–Chitosan (Cu(II)–CS) complex coatings deposited by electrophoretic deposition (EPD) as potential antibacterial candidate to combat microorganisms to reduce implant related infections. The successful deposition of Cu(II)–CS complex coatings on stainless steel was confirmed by physicochemical characterizations. Morphological and elemental analyses by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy verified the uniform distribution of copper in the Chitosan (CS) matrix. Moreover, homogeneous coatings without precipitation of metallic copper were confirmed by X-ray diffraction (XRD) spectroscopy and SEM micrographs. Controlled swelling behavior depicted the chelation of copper with polysaccharide chains that is key to the stability of Cu(II)–CS coatings. All investigated systems exhibited stable degradation rate in phosphate buffered saline (PBS)–lysozyme solution within seven days of incubation. The coatings presented higher mechanical properties with the increase in Cu(II) concentration. The crack-free coatings showed mildly hydrophobic behavior. Antibacterial assays were performed using both Gram-positive and Gram-negative bacteria. Outstanding antibacterial properties of the coatings were confirmed. After 24 h of incubation, cell studies of coatings confirms that up to a certain threshold concentration of Cu(II) were not cytotoxic to human osteoblast-like cells. Overall, our results show that uniform and homogeneous Cu(II)–CS coatings with good antibacterial and enhanced mechanical stability could be successfully deposited by EPD. Such antibiotic-free antibacterial coatings are potential candidates for biomedical implants.
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spelling pubmed-71773502020-04-28 Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings Akhtar, Muhammad Asim Ilyas, Kanwal Dlouhý, Ivo Siska, Filip Boccaccini, Aldo R. Int J Mol Sci Article Bacterial infection associated with medical implants is a major threat to healthcare. This work reports the fabrication of Copper(II)–Chitosan (Cu(II)–CS) complex coatings deposited by electrophoretic deposition (EPD) as potential antibacterial candidate to combat microorganisms to reduce implant related infections. The successful deposition of Cu(II)–CS complex coatings on stainless steel was confirmed by physicochemical characterizations. Morphological and elemental analyses by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy verified the uniform distribution of copper in the Chitosan (CS) matrix. Moreover, homogeneous coatings without precipitation of metallic copper were confirmed by X-ray diffraction (XRD) spectroscopy and SEM micrographs. Controlled swelling behavior depicted the chelation of copper with polysaccharide chains that is key to the stability of Cu(II)–CS coatings. All investigated systems exhibited stable degradation rate in phosphate buffered saline (PBS)–lysozyme solution within seven days of incubation. The coatings presented higher mechanical properties with the increase in Cu(II) concentration. The crack-free coatings showed mildly hydrophobic behavior. Antibacterial assays were performed using both Gram-positive and Gram-negative bacteria. Outstanding antibacterial properties of the coatings were confirmed. After 24 h of incubation, cell studies of coatings confirms that up to a certain threshold concentration of Cu(II) were not cytotoxic to human osteoblast-like cells. Overall, our results show that uniform and homogeneous Cu(II)–CS coatings with good antibacterial and enhanced mechanical stability could be successfully deposited by EPD. Such antibiotic-free antibacterial coatings are potential candidates for biomedical implants. MDPI 2020-04-10 /pmc/articles/PMC7177350/ /pubmed/32290155 http://dx.doi.org/10.3390/ijms21072637 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akhtar, Muhammad Asim
Ilyas, Kanwal
Dlouhý, Ivo
Siska, Filip
Boccaccini, Aldo R.
Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
title Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
title_full Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
title_fullStr Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
title_full_unstemmed Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
title_short Electrophoretic Deposition of Copper(II)–Chitosan Complexes for Antibacterial Coatings
title_sort electrophoretic deposition of copper(ii)–chitosan complexes for antibacterial coatings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177350/
https://www.ncbi.nlm.nih.gov/pubmed/32290155
http://dx.doi.org/10.3390/ijms21072637
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