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Protein Signature in Saliva of Temporomandibular Disorders Myalgia
In the last years, several attempts have been made to study specific biological markers of temporomandibular disorders (TMD). So far, no laboratory tests have been appropriately validated for the diagnosis and prognosis of these disorders. This study aimed to investigate the proteomic profile of the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177369/ https://www.ncbi.nlm.nih.gov/pubmed/32272779 http://dx.doi.org/10.3390/ijms21072569 |
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author | Jasim, Hajer Ernberg, Malin Carlsson, Anders Gerdle, Björn Ghafouri, Bijar |
author_facet | Jasim, Hajer Ernberg, Malin Carlsson, Anders Gerdle, Björn Ghafouri, Bijar |
author_sort | Jasim, Hajer |
collection | PubMed |
description | In the last years, several attempts have been made to study specific biological markers of temporomandibular disorders (TMD). So far, no laboratory tests have been appropriately validated for the diagnosis and prognosis of these disorders. This study aimed to investigate the proteomic profile of the whole stimulated saliva of TMD myalgia patients in order to evaluate potential diagnostic and/or prognostic salivary candidate proteins which could be useful for the management of TMD. Twenty patients diagnosed with TMD myalgia according to the validated Diagnostic Criteria for TMD (DC/TMD) and 20 matched healthy pain-free controls were enrolled. Saliva samples were collected in the morning. Comparative proteomic analysis was performed with two-dimensional gel electrophoresis followed by identification with liquid chromatography–tandem mass spectrometry. Statistical analysis of the quantitative proteomics data revealed that 20 proteins were significantly altered in patients compared to controls. Among these proteins, 12 showed significantly increased levels, and 8 showed significantly decreased levels in patients with TMD myalgia compared to controls. The identified proteins are involved in metabolic processes, immune response, and stress response. This proteomic study shows that the salivary protein profile can discriminate patients with TMD myalgia from healthy subjects, but the protein signature has no correlation with the clinical features of TMD myalgia. Additional studies are needed to validate our observations in additional sample sets and to continue assessing the utility of saliva as a suitable sample for studying processes related to TMD myalgia. |
format | Online Article Text |
id | pubmed-7177369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71773692020-04-28 Protein Signature in Saliva of Temporomandibular Disorders Myalgia Jasim, Hajer Ernberg, Malin Carlsson, Anders Gerdle, Björn Ghafouri, Bijar Int J Mol Sci Article In the last years, several attempts have been made to study specific biological markers of temporomandibular disorders (TMD). So far, no laboratory tests have been appropriately validated for the diagnosis and prognosis of these disorders. This study aimed to investigate the proteomic profile of the whole stimulated saliva of TMD myalgia patients in order to evaluate potential diagnostic and/or prognostic salivary candidate proteins which could be useful for the management of TMD. Twenty patients diagnosed with TMD myalgia according to the validated Diagnostic Criteria for TMD (DC/TMD) and 20 matched healthy pain-free controls were enrolled. Saliva samples were collected in the morning. Comparative proteomic analysis was performed with two-dimensional gel electrophoresis followed by identification with liquid chromatography–tandem mass spectrometry. Statistical analysis of the quantitative proteomics data revealed that 20 proteins were significantly altered in patients compared to controls. Among these proteins, 12 showed significantly increased levels, and 8 showed significantly decreased levels in patients with TMD myalgia compared to controls. The identified proteins are involved in metabolic processes, immune response, and stress response. This proteomic study shows that the salivary protein profile can discriminate patients with TMD myalgia from healthy subjects, but the protein signature has no correlation with the clinical features of TMD myalgia. Additional studies are needed to validate our observations in additional sample sets and to continue assessing the utility of saliva as a suitable sample for studying processes related to TMD myalgia. MDPI 2020-04-07 /pmc/articles/PMC7177369/ /pubmed/32272779 http://dx.doi.org/10.3390/ijms21072569 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jasim, Hajer Ernberg, Malin Carlsson, Anders Gerdle, Björn Ghafouri, Bijar Protein Signature in Saliva of Temporomandibular Disorders Myalgia |
title | Protein Signature in Saliva of Temporomandibular Disorders Myalgia |
title_full | Protein Signature in Saliva of Temporomandibular Disorders Myalgia |
title_fullStr | Protein Signature in Saliva of Temporomandibular Disorders Myalgia |
title_full_unstemmed | Protein Signature in Saliva of Temporomandibular Disorders Myalgia |
title_short | Protein Signature in Saliva of Temporomandibular Disorders Myalgia |
title_sort | protein signature in saliva of temporomandibular disorders myalgia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177369/ https://www.ncbi.nlm.nih.gov/pubmed/32272779 http://dx.doi.org/10.3390/ijms21072569 |
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