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Lessons Learned from Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryoni...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177533/ https://www.ncbi.nlm.nih.gov/pubmed/32230814 http://dx.doi.org/10.3390/ijms21072314 |
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author | Gouveia, Chantel Huyser, Carin Egli, Dieter Pepper, Michael S. |
author_facet | Gouveia, Chantel Huyser, Carin Egli, Dieter Pepper, Michael S. |
author_sort | Gouveia, Chantel |
collection | PubMed |
description | Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic cloning, or for the purpose of reproductive cloning. However, the consensus is that the low efficiency in creating normal viable offspring in animals by SCNT (1–5%) and the high number of abnormalities seen in these cloned animals is due to epigenetic reprogramming failure. In this review we provide an overview of the current literature on SCNT, focusing on protocol development, which includes early SCNT protocol deficiencies and optimizations along with donor cell type and cell cycle synchrony; epigenetic reprogramming in SCNT; current protocol optimizations such as nuclear reprogramming strategies that can be applied to improve epigenetic reprogramming by SCNT; applications of SCNT; the ethical and legal implications of SCNT in humans; and specific lessons learned for establishing an optimized SCNT protocol using a mouse model. |
format | Online Article Text |
id | pubmed-7177533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71775332020-04-28 Lessons Learned from Somatic Cell Nuclear Transfer Gouveia, Chantel Huyser, Carin Egli, Dieter Pepper, Michael S. Int J Mol Sci Review Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic cloning, or for the purpose of reproductive cloning. However, the consensus is that the low efficiency in creating normal viable offspring in animals by SCNT (1–5%) and the high number of abnormalities seen in these cloned animals is due to epigenetic reprogramming failure. In this review we provide an overview of the current literature on SCNT, focusing on protocol development, which includes early SCNT protocol deficiencies and optimizations along with donor cell type and cell cycle synchrony; epigenetic reprogramming in SCNT; current protocol optimizations such as nuclear reprogramming strategies that can be applied to improve epigenetic reprogramming by SCNT; applications of SCNT; the ethical and legal implications of SCNT in humans; and specific lessons learned for establishing an optimized SCNT protocol using a mouse model. MDPI 2020-03-27 /pmc/articles/PMC7177533/ /pubmed/32230814 http://dx.doi.org/10.3390/ijms21072314 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Gouveia, Chantel Huyser, Carin Egli, Dieter Pepper, Michael S. Lessons Learned from Somatic Cell Nuclear Transfer |
title | Lessons Learned from Somatic Cell Nuclear Transfer |
title_full | Lessons Learned from Somatic Cell Nuclear Transfer |
title_fullStr | Lessons Learned from Somatic Cell Nuclear Transfer |
title_full_unstemmed | Lessons Learned from Somatic Cell Nuclear Transfer |
title_short | Lessons Learned from Somatic Cell Nuclear Transfer |
title_sort | lessons learned from somatic cell nuclear transfer |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177533/ https://www.ncbi.nlm.nih.gov/pubmed/32230814 http://dx.doi.org/10.3390/ijms21072314 |
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