Cargando…

Lessons Learned from Somatic Cell Nuclear Transfer

Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryoni...

Descripción completa

Detalles Bibliográficos
Autores principales: Gouveia, Chantel, Huyser, Carin, Egli, Dieter, Pepper, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177533/
https://www.ncbi.nlm.nih.gov/pubmed/32230814
http://dx.doi.org/10.3390/ijms21072314
_version_ 1783525240910905344
author Gouveia, Chantel
Huyser, Carin
Egli, Dieter
Pepper, Michael S.
author_facet Gouveia, Chantel
Huyser, Carin
Egli, Dieter
Pepper, Michael S.
author_sort Gouveia, Chantel
collection PubMed
description Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic cloning, or for the purpose of reproductive cloning. However, the consensus is that the low efficiency in creating normal viable offspring in animals by SCNT (1–5%) and the high number of abnormalities seen in these cloned animals is due to epigenetic reprogramming failure. In this review we provide an overview of the current literature on SCNT, focusing on protocol development, which includes early SCNT protocol deficiencies and optimizations along with donor cell type and cell cycle synchrony; epigenetic reprogramming in SCNT; current protocol optimizations such as nuclear reprogramming strategies that can be applied to improve epigenetic reprogramming by SCNT; applications of SCNT; the ethical and legal implications of SCNT in humans; and specific lessons learned for establishing an optimized SCNT protocol using a mouse model.
format Online
Article
Text
id pubmed-7177533
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71775332020-04-28 Lessons Learned from Somatic Cell Nuclear Transfer Gouveia, Chantel Huyser, Carin Egli, Dieter Pepper, Michael S. Int J Mol Sci Review Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic cloning, or for the purpose of reproductive cloning. However, the consensus is that the low efficiency in creating normal viable offspring in animals by SCNT (1–5%) and the high number of abnormalities seen in these cloned animals is due to epigenetic reprogramming failure. In this review we provide an overview of the current literature on SCNT, focusing on protocol development, which includes early SCNT protocol deficiencies and optimizations along with donor cell type and cell cycle synchrony; epigenetic reprogramming in SCNT; current protocol optimizations such as nuclear reprogramming strategies that can be applied to improve epigenetic reprogramming by SCNT; applications of SCNT; the ethical and legal implications of SCNT in humans; and specific lessons learned for establishing an optimized SCNT protocol using a mouse model. MDPI 2020-03-27 /pmc/articles/PMC7177533/ /pubmed/32230814 http://dx.doi.org/10.3390/ijms21072314 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gouveia, Chantel
Huyser, Carin
Egli, Dieter
Pepper, Michael S.
Lessons Learned from Somatic Cell Nuclear Transfer
title Lessons Learned from Somatic Cell Nuclear Transfer
title_full Lessons Learned from Somatic Cell Nuclear Transfer
title_fullStr Lessons Learned from Somatic Cell Nuclear Transfer
title_full_unstemmed Lessons Learned from Somatic Cell Nuclear Transfer
title_short Lessons Learned from Somatic Cell Nuclear Transfer
title_sort lessons learned from somatic cell nuclear transfer
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177533/
https://www.ncbi.nlm.nih.gov/pubmed/32230814
http://dx.doi.org/10.3390/ijms21072314
work_keys_str_mv AT gouveiachantel lessonslearnedfromsomaticcellnucleartransfer
AT huysercarin lessonslearnedfromsomaticcellnucleartransfer
AT eglidieter lessonslearnedfromsomaticcellnucleartransfer
AT peppermichaels lessonslearnedfromsomaticcellnucleartransfer