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Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation

Jabara (Citrus jabara Hort. ex Y. Tanaka) is a type of citrus fruit known for its beneficial effect against seasonal allergies. Jabara is rich in the antioxidant narirutin whose anti-allergy effect has been demonstrated. One of the disadvantages in consuming Jabara is its bitter flavor. Therefore, w...

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Autores principales: Uchida, Ryota, Kato, Michiko, Hattori, Yuka, Kikuchi, Hiroko, Watanabe, Emi, Kobayashi, Katsuumi, Nishida, Keigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177689/
https://www.ncbi.nlm.nih.gov/pubmed/32252468
http://dx.doi.org/10.3390/ijms21072472
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author Uchida, Ryota
Kato, Michiko
Hattori, Yuka
Kikuchi, Hiroko
Watanabe, Emi
Kobayashi, Katsuumi
Nishida, Keigo
author_facet Uchida, Ryota
Kato, Michiko
Hattori, Yuka
Kikuchi, Hiroko
Watanabe, Emi
Kobayashi, Katsuumi
Nishida, Keigo
author_sort Uchida, Ryota
collection PubMed
description Jabara (Citrus jabara Hort. ex Y. Tanaka) is a type of citrus fruit known for its beneficial effect against seasonal allergies. Jabara is rich in the antioxidant narirutin whose anti-allergy effect has been demonstrated. One of the disadvantages in consuming Jabara is its bitter flavor. Therefore, we fermented the fruit to reduce the bitterness and make Jabara easy to consume. Here, we examined whether fermentation alters the anti-allergic property of Jabara. Suppression of degranulation and cytokine production was observed in mast cells treated with fermented Jabara and the effect was dependent on the length of fermentation. We also showed that 5-hydroxymethylfurfural (5-HMF) increases as fermentation progresses and was identified as an active component of fermented Jabara, which inhibited mast cell degranulation. Mast cells treated with 5-HMF also exhibited reduced degranulation and cytokine production. In addition, we showed that the expression levels of phospho-PLCγ1 and phospho-ERK1/2 were markedly reduced upon FcεRI stimulation. These results indicate that 5-HMF is one of the active components of fermented Jabara that is involved in the inhibition of mast cell activation.
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spelling pubmed-71776892020-04-28 Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation Uchida, Ryota Kato, Michiko Hattori, Yuka Kikuchi, Hiroko Watanabe, Emi Kobayashi, Katsuumi Nishida, Keigo Int J Mol Sci Article Jabara (Citrus jabara Hort. ex Y. Tanaka) is a type of citrus fruit known for its beneficial effect against seasonal allergies. Jabara is rich in the antioxidant narirutin whose anti-allergy effect has been demonstrated. One of the disadvantages in consuming Jabara is its bitter flavor. Therefore, we fermented the fruit to reduce the bitterness and make Jabara easy to consume. Here, we examined whether fermentation alters the anti-allergic property of Jabara. Suppression of degranulation and cytokine production was observed in mast cells treated with fermented Jabara and the effect was dependent on the length of fermentation. We also showed that 5-hydroxymethylfurfural (5-HMF) increases as fermentation progresses and was identified as an active component of fermented Jabara, which inhibited mast cell degranulation. Mast cells treated with 5-HMF also exhibited reduced degranulation and cytokine production. In addition, we showed that the expression levels of phospho-PLCγ1 and phospho-ERK1/2 were markedly reduced upon FcεRI stimulation. These results indicate that 5-HMF is one of the active components of fermented Jabara that is involved in the inhibition of mast cell activation. MDPI 2020-04-02 /pmc/articles/PMC7177689/ /pubmed/32252468 http://dx.doi.org/10.3390/ijms21072472 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Uchida, Ryota
Kato, Michiko
Hattori, Yuka
Kikuchi, Hiroko
Watanabe, Emi
Kobayashi, Katsuumi
Nishida, Keigo
Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
title Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
title_full Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
title_fullStr Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
title_full_unstemmed Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
title_short Identification of 5-Hydroxymethylfurfural (5-HMF) as an Active Component Citrus Jabara That Suppresses FcεRI-Mediated Mast Cell Activation
title_sort identification of 5-hydroxymethylfurfural (5-hmf) as an active component citrus jabara that suppresses fcεri-mediated mast cell activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177689/
https://www.ncbi.nlm.nih.gov/pubmed/32252468
http://dx.doi.org/10.3390/ijms21072472
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