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Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen

Microorganisms that create mixed-species biofilms in the human oral cavity include, among others, the opportunistic fungus Candida albicans and the key bacterial pathogen in periodontitis, Porphyromonas gingivalis. Both species use arsenals of virulence factors to invade the host organism and evade...

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Autores principales: Karkowska-Kuleta, Justyna, Surowiec, Magdalena, Gogol, Mariusz, Koziel, Joanna, Potempa, Barbara, Potempa, Jan, Kozik, Andrzej, Rapala-Kozik, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177930/
https://www.ncbi.nlm.nih.gov/pubmed/32260245
http://dx.doi.org/10.3390/ijms21072495
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author Karkowska-Kuleta, Justyna
Surowiec, Magdalena
Gogol, Mariusz
Koziel, Joanna
Potempa, Barbara
Potempa, Jan
Kozik, Andrzej
Rapala-Kozik, Maria
author_facet Karkowska-Kuleta, Justyna
Surowiec, Magdalena
Gogol, Mariusz
Koziel, Joanna
Potempa, Barbara
Potempa, Jan
Kozik, Andrzej
Rapala-Kozik, Maria
author_sort Karkowska-Kuleta, Justyna
collection PubMed
description Microorganisms that create mixed-species biofilms in the human oral cavity include, among others, the opportunistic fungus Candida albicans and the key bacterial pathogen in periodontitis, Porphyromonas gingivalis. Both species use arsenals of virulence factors to invade the host organism and evade its immune system including peptidylarginine deiminase that citrullinates microbial and host proteins, altering their function. We assessed the effects of this modification on the interactions between the C. albicans cell surface and human plasminogen and kininogen, key components of plasma proteolytic cascades related to the maintenance of hemostasis and innate immunity. Mass spectrometry was used to identify protein citrullination, and microplate tests to quantify the binding of modified plasminogen and kininogen to C. albicans cells. Competitive radioreceptor assays tested the affinity of citrullinated kinins to their specific cellular receptors. The citrullination of surface-exposed fungal proteins reduced the level of unmodified plasminogen binding but did not affect unmodified kininogen binding. However, the modification of human proteins did not disrupt their adsorption to the unmodified fungal cells. In contrast, the citrullination of kinins exerted a significant impact on their interactions with cellular receptors reducing their affinity and thus affecting the role of kinin peptides in the development of inflammation.
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spelling pubmed-71779302020-04-28 Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen Karkowska-Kuleta, Justyna Surowiec, Magdalena Gogol, Mariusz Koziel, Joanna Potempa, Barbara Potempa, Jan Kozik, Andrzej Rapala-Kozik, Maria Int J Mol Sci Article Microorganisms that create mixed-species biofilms in the human oral cavity include, among others, the opportunistic fungus Candida albicans and the key bacterial pathogen in periodontitis, Porphyromonas gingivalis. Both species use arsenals of virulence factors to invade the host organism and evade its immune system including peptidylarginine deiminase that citrullinates microbial and host proteins, altering their function. We assessed the effects of this modification on the interactions between the C. albicans cell surface and human plasminogen and kininogen, key components of plasma proteolytic cascades related to the maintenance of hemostasis and innate immunity. Mass spectrometry was used to identify protein citrullination, and microplate tests to quantify the binding of modified plasminogen and kininogen to C. albicans cells. Competitive radioreceptor assays tested the affinity of citrullinated kinins to their specific cellular receptors. The citrullination of surface-exposed fungal proteins reduced the level of unmodified plasminogen binding but did not affect unmodified kininogen binding. However, the modification of human proteins did not disrupt their adsorption to the unmodified fungal cells. In contrast, the citrullination of kinins exerted a significant impact on their interactions with cellular receptors reducing their affinity and thus affecting the role of kinin peptides in the development of inflammation. MDPI 2020-04-03 /pmc/articles/PMC7177930/ /pubmed/32260245 http://dx.doi.org/10.3390/ijms21072495 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Karkowska-Kuleta, Justyna
Surowiec, Magdalena
Gogol, Mariusz
Koziel, Joanna
Potempa, Barbara
Potempa, Jan
Kozik, Andrzej
Rapala-Kozik, Maria
Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen
title Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen
title_full Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen
title_fullStr Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen
title_full_unstemmed Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen
title_short Peptidylarginine Deiminase of Porphyromonas gingivalis Modulates the Interactions between Candida albicans Biofilm and Human Plasminogen and High-Molecular-Mass Kininogen
title_sort peptidylarginine deiminase of porphyromonas gingivalis modulates the interactions between candida albicans biofilm and human plasminogen and high-molecular-mass kininogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177930/
https://www.ncbi.nlm.nih.gov/pubmed/32260245
http://dx.doi.org/10.3390/ijms21072495
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