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Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain

Endometriosis is a prevalent gynecologic disease, defined by dysfunctional endometrium-like lesions outside of the uterine cavity. These lesions are presumably established via retrograde menstruation, i.e., endometrial tissue that flows backwards during menses into the abdomen and deposits on the or...

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Autores principales: Persoons, Eleonora, De Clercq, Katrien, Van den Eynde, Charlotte, Pinto, Sílvia João Poseiro coutinho, Luyten, Katrien, Van Bree, Rita, Tomassetti, Carla, Voets, Thomas, Vriens, Joris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177935/
https://www.ncbi.nlm.nih.gov/pubmed/32230898
http://dx.doi.org/10.3390/ijms21072326
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author Persoons, Eleonora
De Clercq, Katrien
Van den Eynde, Charlotte
Pinto, Sílvia João Poseiro coutinho
Luyten, Katrien
Van Bree, Rita
Tomassetti, Carla
Voets, Thomas
Vriens, Joris
author_facet Persoons, Eleonora
De Clercq, Katrien
Van den Eynde, Charlotte
Pinto, Sílvia João Poseiro coutinho
Luyten, Katrien
Van Bree, Rita
Tomassetti, Carla
Voets, Thomas
Vriens, Joris
author_sort Persoons, Eleonora
collection PubMed
description Endometriosis is a prevalent gynecologic disease, defined by dysfunctional endometrium-like lesions outside of the uterine cavity. These lesions are presumably established via retrograde menstruation, i.e., endometrial tissue that flows backwards during menses into the abdomen and deposits on the organs. As ongoing pain is one of the main pain symptoms of patients, an animal model that illuminates this problem is highly anticipated. In the present study, we developed and validated a rat model for ongoing endometriosis-associated pain. First, menstrual endometrial tissue was successfully generated in donor rats, as validated by gross examination, histology and qPCR. Next, endometriosis was induced in recipient animals by intraperitoneal injection of menstrual tissue. This resulted in neuro-angiogenesis as well as established endometriosis lesions, which were similar to their human counterparts, since epithelial and stromal cells were observed. Furthermore, significant differences were noted between control and endometriosis animals concerning bodyweight and posture changes, indicating the presence of ongoing pain in animals with endometriosis. In summary, a rat model for endometriosis was established that reliably mimics the human pathophysiology of endometriosis and in which signs of ongoing pain were detected, thus providing a new research tool for therapy development.
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spelling pubmed-71779352020-04-28 Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain Persoons, Eleonora De Clercq, Katrien Van den Eynde, Charlotte Pinto, Sílvia João Poseiro coutinho Luyten, Katrien Van Bree, Rita Tomassetti, Carla Voets, Thomas Vriens, Joris Int J Mol Sci Article Endometriosis is a prevalent gynecologic disease, defined by dysfunctional endometrium-like lesions outside of the uterine cavity. These lesions are presumably established via retrograde menstruation, i.e., endometrial tissue that flows backwards during menses into the abdomen and deposits on the organs. As ongoing pain is one of the main pain symptoms of patients, an animal model that illuminates this problem is highly anticipated. In the present study, we developed and validated a rat model for ongoing endometriosis-associated pain. First, menstrual endometrial tissue was successfully generated in donor rats, as validated by gross examination, histology and qPCR. Next, endometriosis was induced in recipient animals by intraperitoneal injection of menstrual tissue. This resulted in neuro-angiogenesis as well as established endometriosis lesions, which were similar to their human counterparts, since epithelial and stromal cells were observed. Furthermore, significant differences were noted between control and endometriosis animals concerning bodyweight and posture changes, indicating the presence of ongoing pain in animals with endometriosis. In summary, a rat model for endometriosis was established that reliably mimics the human pathophysiology of endometriosis and in which signs of ongoing pain were detected, thus providing a new research tool for therapy development. MDPI 2020-03-27 /pmc/articles/PMC7177935/ /pubmed/32230898 http://dx.doi.org/10.3390/ijms21072326 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Persoons, Eleonora
De Clercq, Katrien
Van den Eynde, Charlotte
Pinto, Sílvia João Poseiro coutinho
Luyten, Katrien
Van Bree, Rita
Tomassetti, Carla
Voets, Thomas
Vriens, Joris
Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain
title Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain
title_full Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain
title_fullStr Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain
title_full_unstemmed Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain
title_short Mimicking Sampson’s Retrograde Menstrual Theory in Rats: A New Rat Model for Ongoing Endometriosis-Associated Pain
title_sort mimicking sampson’s retrograde menstrual theory in rats: a new rat model for ongoing endometriosis-associated pain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177935/
https://www.ncbi.nlm.nih.gov/pubmed/32230898
http://dx.doi.org/10.3390/ijms21072326
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